Studies On Processing Technology And Quality Standards Of Euphorbia Ebracteolata Hayata By Vinegar

This postgraduate degree thesis was supported by special foundation of State Administra-tion of Traditional Chinese Medicine,"Processing technology and mechanism of the toxic tra-dition Chinese medicines and the ones processed by bran "(NO.200807039).At present, the processing technology of Stellera chamaejasme in vinegar is made give pri-ority to, but the lack of specific quantitative parameters of processing technology, to a finished appearance traits as the only reference index.Literature research and preliminary experimental results show that, in which benzene ethyl ketone is its resistance to tuberculosis of the main ef-fective components, including fischeriana B, C in a..After processing the content increased, ter-pene lactone in most toxic, after processing the content decreased.Stellera chamaejasme health products intragastric administration on mouse gastric irritation studies showed that, after proc-essing on gastric mucosal irritation reducing PGE2content decreased, mouse gastric body. This thesis on Stellera chamaejasme vinegar processing and chemical composition, pharmacodynam-ics and toxicity were studied by orthogonal experiments, the vinegar, the processing technology of Stellera chamaejasme was optimized, and on this basis set Stellera chamaejasme quality stan-dards of medicinal vinegar.In two, total terpene lactones of Stellera chamaejasme B,C peptide of Stellera chamaejasme and after intragastric administration of mouse stomach body PGE2content as determined the optimal processing technology indicators, using L9(34) orthogonal test method, the processing of materials of Stellera chamaejasme amount, roasting temperature and roasting time and other factors were studied, screening out the best conditions of Stellera chamaejasme pieces concocted vinegar. Main research contents:1.Comparative study on.chemical composition of crude and processed euphorbia ehracte-olata radixTo research the variation of chemical constituents in Euphorbia ebracteolata Hayata after processing. a colorimetric method was established for the assay of the total lactones as the toxic components with Kedde as the coloring reagent and measuring of the absorbance with U V, de-termine the content of ebracteolata cpd B and ebracteolata cpd C as active ingredients and estab-lish the characteristics map. After processing,the content of the total lactone was reduced,the content of ebracteolata cpd B and ebracteolata cpd C was increased, the characteristics map has rich informations,can reflect the law of the overall chang after processing. After processing, The chemical composition of Euphorbia ebracteolata Hayata has a significant chang, the increased efficacy and reduced toxicity may related to changes in composition.2. Comparative study on efficacy and toxicity of crude and processed euphorbia ehracteo-lata radixTo compare the toxicity from euphorbia before and after processing. From the pathological examination and analyzed the weight and PGE2contents of mice’s digestive system and con-junctival irritation test of rabbits’ eyes after administration of euphorbia. The comparative stuty show that processed Euphorbia ebracteolata Hayata irritating toxic become lower than crude euphorbia. This best processing techinique is stable,feasible and good repetition.3.Optimization of processing technique of euphorbia ehracteolata radix praeparatum cun vinegrTo establish the best processing technique of Euphorbia ebracteolata Hayata. The best proc-essing technique was gained through determine the content of total lactones as the toxic com-ponents and determine the content of ebracteolata cpd B and ebracteolata cpd C as active ingre-dients and PGE2contents of mice’s digestive system after administration of euphorbia as in-dex,and orthogonal design experiments L9(34) was used to investigate the factors of vinegar amount baked time and temperature. The best processing technique was to add40,vinegar and bake10minutes at160.C. This best processing techinique is stable,feasible and good repeti-tion..4. The establishment of standards of euphorbia ehracteolata radix praeparatum cun vinegrCollection of the national different sources of10batches of vinegar of Stellera chamaejasme pieces, wherein the3batch of Jiangsu Haichang Herbal Pieces Factory according to the labora-tory the optimized processing technology try to enlarge the production of standard pieces of Stellera chamaejasme in vinegar.According to the quality standard system, the pieces of the character identification, examination and determination of content of comprehensive research, established the vinegar of Stellera chamaejasme pieces objective, quantitative quality evaluation standard, and the drafting of vinegar of Stellera chamaejasme quality standard draft.