Effects Of Prostaglandin E1on Apoptosis And The Expression Of Apoptosis-related Proteins In Hepatic Of Rats With Obstructive Jaundice

Objective To investigate the effect of Prostaglandin E1on apoptosis in hepatic of ratswith obstructive jaundice （OJ）. Understanding the relationship between liver cellapoptosis of rats with obstructive jaundice and the expression of apoptosis-related genesiNOS/NF-κB.Methods Seventy-two health Wistar rats were divided into three groups randomly:sham operation group （SH group）, double-ligating bile duct group （BDL group）and thegroup of BDL rats administered Prostaglandin E1（PGE₁group）. Sham operation group,the common bile duct was only exposed and not ligation. Completion of the preparationof animal model, PGE₁group were given tail vein injection of PGE₁3μg.kg^-1, the SHgroup and BDL group were given the capacity of the tail vein injection of0.9%NaCl.Three groups of rats were sacrificed on the3,7and10days of postoperationrespectively. The levels of serum total bilirubin （TBil）, direct bilirubin （DBil）, alanineaminotransferase（ALT） and aspartate aminotransferase（AST） were tested and thetissue’s histopathology changes were observed；Apoptosis of hepatocytes was tested byTUNEL and accounted apoptosis index（AI）, the immunohistochemical technique wasused for detecting the NF-κB and iNOS protein expression.Results1. With the SH group at the same phase points, the BDL group serum of AST, ALT,TBIL, DBIL were significantly increased in postoperative3d,7d,10d day, the difference was statistically significant （P <0.01）. Compared with the BDL group,PGE₁group serum liver function of TBil DBil level had not statistically significanceafter three postoperative; While the serum of ALT, AST, had statistically significantdifference （P <0.05）. And differences of serum of AST, ALT,, TBIL, DBIL levelwere statistically significant （P <0.05） after7d and10d of operation.2. With the SH group at the same phase points, the apoptotic index of rat hepatocytesof the BDL group was significantly increased （P <0.01）.With the extension of thedays of bile duct ligation, the apoptosis was more obvious. Compared with the BDLgroup at the time points, PGE₁group rat hepatocytes apoptosis index wassignificantly less （P <0.01）.3. SH rats liver cell morphology change was not significant, BDL rats liver cells wereseen within the different degrees of cholestasis and/or cell and inflammatory cellinfiltration, and with bile duct expansion apoptosis become increasingly apparentwith time. While the change of PGE₁group rats at different time points was lessthan the BDL group.4. With the SH group at the same phase points, the expression of iNOS/NF-κB inBDL group liver tissue were significantly increased （P <0.01）. Compared with theBDL group at the time points, the lower expression of iNOS and higher expressionof NF-κB of rat liver tissue in PGE₁group （P <0.05, P <0.01）.Conclusion Rats with common bile duct ligation induced cholestasis can causeobviously damage to the liver function and liver cell apoptosis.PGE₁can ease thedegree of hepatic functional injury in rats with obstructive jaundice, for it may directlyor indirectly decrease cell apoptosis by up-regulating the expression of NF-κB anddown-regulating the expression of iNOS in hepatic tissue.