The Expression And Significance Of GOLPH3and Mtor In Gastrointestinal Stromal Tumors

Background and Objective:The Golgi phosphoprotein3(GOLPH3) has recently been identified as oncegene,which could promote the growth,differentiation and proliferation of tumor cell by regulating and controling the mammalian target of rapamycin(mTOR) signal pathway.It also regulates the susceptibilities of tumor cells to rapamycin. The studies on expression of GOLPH3in cancer are relatively scarce, that has no in gastrointestinal stromal tumors(GISTs) at internal and abroad at present.In the present study, expression of GOLPH3and mTOR was detected, the relationship between GOLPIH3、mTOR and GISTs clinical pathological indicators; the role they played as an assistant index in risk degree classification,the possibility of new target for GISTs molecular targeted therapy were discussed.Materials and Method:Clinical and pathological data were retrospectively analyzed.SP immunohistochemistry was used to detect the expression level of GOLPH3and mTOR in48samples of GISTs tissue,10cases of normal gastric tissues tissues and10cases of normal intestinal tissue. In addition, NovoLinkTM polymer inspection system was used to detect the expression of DOG-1、CD117、CD34、Desmin、α-SMA、 S-100in48samples of GISTs which Used in the diagnosis of GISTs again. The rank-sum test and Kendall rank correlation analysis was used to analyze the relation between GOLPH3、mTOR and GISTs clinic pathological factors. Results:1、he expression of GOLPH3and mTOR in GISTs was significantly higher than that in normal gastrointestinal tissue. There is a significant difference statistically (x2=21.596, x2=32.788,P<0.05);2、 The positive intensity of GOLPH3expression in GISTs strengthens with nuclear fission, tumor size, risk(r=0.361、r=0.389、r=0.434, P<0.05),and there was significant difference between these factors (x2=7.692, x2=9.572, x2=12.579、P<0.05), But the expression differences between the groups in gender, age, tumor growth parts, organization type, tumor necrosis, tumor rupture have no statistically significant;3、The expression of mTOR in GISTs differences between the groups of gender, age, tumor growth parts, organization type, tumor necrosis, tumor rupture, nuclear fission, tumor size and the risk have no statistically significant (p>0.05);4、The expression of GOLPH3and mTOR in GISTs have no Significant relationship with DOG-1, CD117, CD34, Desmin, α-SMA and S-100;5、 GOLPH3is positively associated with the expression of mTOR in GISTs (r=0.293, P<0.05)Conclusion:1、The positive rate of GOLPH3&mTOR in GISTs is significantly higher than that of normal tissue. It indicates that aberrant GOLPH3&mTOR expression is closely related to GIST.2、The positive intensity of GOLPH3expression in GISTs strengthens with nuclear fission,tumor size,risk.It suggest the GOLPH3expression increases with GISTs malignancy,which may be a marker that judge the malignant degree. 3、The expression level of mTOR is positively associated with GOLPH3.It suggest that GOLPH3take participate in the process of carcinogenesis and progression GISTs by mTOR signaling pathways.4、The high expression suggests GOLPH3can be a new target for GISTs molecular targeted therapy...