| Objective:To probe into making of animal model of disharmony of TCM type between liver and stomach by observing changes of ordinary situations, weights, behavior and gastrointestinal hormone conce-ntrations in blood based on pertinence of disharmony of TCM type between liver and stomach and "depression-functional dyspepsia". Investigate the pathogenesis of disharmony of TCM type between liver and stomach by observing changes of the items above with Shugan Hewei Decoction (SHD)Methods:The rats were treated by Chronic Unpredictable Mild Stress (CUMS) and separation according to related references. Divide the one hundred and twenty SPF Wistar rats into five groups randomly, the normal saline group, model group, Fluoxetine group and the high, low dosage of SHD groups. Begin to give medication after two weeks of the establishment of the model. Fluoxetine group and high, low dosage of SHD group and were given Fluoxetine and high, low dosage of SHD by0.36mg/kg,20g/kg and10g/kg in weights through introgastric administration respectively, once a day; the normal saline group and model group were given the same volume physiol-ogical saline. Every group must give continuous course treatment for fourteen days. On the second, third and fourth week of the establishment of the model,observe ordinary situations, weights and behavior changes of model rats and detect gastrin (GAS), motilin (MTL), vasoactive intestinal peptide (VIP) and substance P (SP) concentrations in their blood serum by methods of enzyme linked immuno sorbent assay (ELISA).Results:1Ordinary situations:On the second week of the establishment of the model, the conditions of the rats in the normal saline group were good. Hair of the model rats were disordered and weights, intake of food and drinking water were descendent. And their frequency of resistance lowered when snatched. On the third week, the conditions of the rats in the normal saline group were good, too. The model rats were calm after stimulation and their frequency of resistance lowered obviously even stop struggling. Weights, intake of food and drinking water were descendent obviously as well. On the fourth week, the conditions of the rats in the normal saline group were still good. Hair of the model rats were lost sheen, easily chipped off. They were inactive, tended to flock together, lagged in response and stopped struggling when snatched. Weights, intake of food and drinking water were descendent very obviously. The syndromes of all the medication groups had been improved slightly after treated for seven days; the syndromes of all the medication groups had been improved evidently for fourteen days, especially the high dosage of SHD.2Changes of weights:Weights of model rats tended to lower on the second week of the establishment of the model (P>0.05), compared to the normal saline group; on the third week, there was a significant difference in weights between the model group and the normal saline group (P<0.05); on the fourth week, there was a very significant difference(P<0.01), which implied that weights of depression model rats decl ined. On the seventh day of treatment, weights of all the medication groups rised. There was a significant difference in weights between all the medication groups and the model group (P<0.05); On the fourteenth day of treatment, that of all the medication groups rised obviously. There was a very significant difference in weights between all the medication groups and the model group (P<0.01), which implied SHD had anti-depression effect. There was a significant difference between the high dosage of SHD group and the Fluoxetine group (P<0.05), which implied action of the high dosage of SHD was better.3Open box experiment:The crossing, rearing and grooming scores of model rats tended to lower, and dejecta number scores rised on the second week of the establishment of the model (P>0.05), compared to the normal saline group; on the third week, there was a significant difference between the model group and the normal sal ine group (P<0.05); on the fourth week, there was a very significant difference (P<0.01), which implied that crossing, rearing and grooming scores of depression model rats declined and dejecta number scores rised. On the seventh day of treatment, crossing, rearing, grooming and dejecta number scores of all the medication groups were improved. There was a significant difference in the crossing, rearing, grooming and dejecta number scores of model rats between all the medication groups and the model group (P<0.05); On the fourteenth day of treatment, that of all the medication groups rised obviously. There was a very significant difference in that of model rats between all the medication groups and the model group (P<0.01), which implied SHD had anti-depression effect. There was a significant difference between the high dosage of SHD group and the Fluoxetine group (P<0.05), which implied action of the high dosage of SHD was better.4The wastages of1%saccharose experiment:The wastages of1%saccharose solution of model rats tended to lower on the second week of the establishment of the model rats (P>0.05), compared to the normal sal ine group; on the third week, there was a significant difference in the wastages of1%saccharose solution between the model group and the normal saline group (P<0.05); on the fourth week, there was a very significant difference(P<0.05or P<0.01), which implied that the wastages of1%saccharose solution of depression model rats declined. On the seventh day of treatment, the wastages of1%saccharose solution of all the medication groups were increased. There was a significant difference in the wastages of1%saccharose solution between all the medication groups and the model group (P<0.05); On the fourteenth day of treatment, that of all the medication groups rised obviously. There was a very significant difference in that between all the medication groups and the model group (P<0.01), which implied SHD had anti-depression effect. There was a significant difference betwe-en the high dosage of SHD group and the Fluoxetine group (P<0.05), which implied action of the high dosage of SHD and Fluoxetine were similar.5Gastrointestinal hormone concentrations in blood:The GAS, MTL and SP concentrations in blood of model rats tended to lower, VIP concentrations in blood rise on the second week of the esta-blishment of the model (P>0.05), compared to the normal saline group; on the third week, there was a significant difference in the GAS, MTL, SP and VIP concentrations in blood between the model group and the normal saline group (P<0.05); on the fourth week, there was a very significant difference (P<0.01), which implied that the GAS, MTL and SP in blood of depression model rats declined and VIP concentrations rised. On the seventh day of treatment, the GAS, MTL, SP and VIP concentrations in blood of all the medication groups were increased. There was a significant difference in the GAS, MTL, SP and VIP concentrations in blood of model rats between all the medication groups and the model group (P<0.05or P<0.01); On the fourteenth day of treatment, that of all the medication groups rised obviously. There was a very significant difference in that of model rats between all the medication groups and the model group (P<0.05or P<0.01), which implied SHD had anti-depression effect. There was a significant difference between the high dosage of SHD group and the Fluoxetine group (P<0.05or P<0.01), which implied action of the high dosage of SHD was better.Conclusion:1The experiment observed dynamic changes of ordinary situati-ons, weights, behavior, GAS, MTL, SP and VIP concentrations in blood serum, which implied that the depression model displayed changes of gastrointestine. It was similar to the model of dishar-mony of TCM type between liver and stomach.2The experiment pointed out changes of gastrointestinal h- ormone in blood serum was related to the pathogenesis of disharmony of TCM type between liver and stomach; SHD could adjust the gastr-ointestinal hormone in blood serum, which may be its therapeutic mechanisms of disharmony of TCM type between liver and stomach. |
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