Analysis Of Intestinal Bacteiral Communities In Four Group Abnormal Hilit Hyperpietics And Normal Hilit Of Uighur Nationality

Objective：The microbial diversity and differences of four abnormal hilit hyperpietics andnormal hilit group was investigated using nested PCR–denaturing gradient gel electrophoresis.Methods:(1) The total DNA in the fecal speciments of abnormal Sawda hilit with (20samples)and normal Sawda hilit healthy people (20samples), abnormal Khan hilit with (20samples)and normal Khan healthy people (20samples), abnormal Sapra hilit with (10samples) andnormal Sapra hilit healthy people (10samples), abnormal Balgham hilit with (10samples) andnormal Balgham hilit healthy people (10samples), total120subjects were used as templates.After the16SrDNA genes (V6-V8region) were amplified by using the universal primers andanalyzed by DGGE. Several bands in DGGE profile specifically existed in two group laneswere excised, cloned, sequenced and searched in BLAST to find the closest relatives.(2) Eachband of DGGE and the similarity of UPGMA cluster were analysized wsed Quantity One4.4.0,and the diversity, richness and evenness of intestinal microflora of bacterial diversity wereanalysized used Microsoft Excel and SPSS17.0.(3) Construct phylogenetic trees of intestinalflora by MEGA5.05software. Results：(1) The results from UPGAMA cluster showed that thesimilarity of total intestinal tract bacteria. Abnormal Sawda hilit hyperpietics(45%-87%)＞abnormal Khan hilit hyperpietics(50-85%)＞abnormal Balgham hilit hyperpietics(42%-86%)＞abnormal Sapra hilit hyperpietics(54-82%), normal Sawda hilit(40%-80%)＞normalKhan hilit(49%-79%)＞normal Balgham hilit(43%-72%)＞normal Sapra hilit(52%-75%).(2) By analyzing the diversity, richness and evenness of four groups of total intestinal bacteria,abnormal Khan hilit hyperpietics and normal Khan hilit, abnormal Balgham hilit hyperpieticsand normal Balgham hilit was statistically significant (P＜0.05). Abnormal Sawda hilithyperpietics respectively compared with abnormal Khan hilit hyperpietics, abnormal Balgham hilit hyperpietics was statistically significant (P＜0.05). Compared with abnormal Sapra hilit,the richness and diversity of total intestinal bacteria was statistically significant (P＜0.05), butthe evenness was no significant (P＞0.05).(3) The sequences that frequency much canrepresentative each sample were compared with sequences in the Genebank nucleotidesequence database by using Blast software and phylogenetic trees were constructed.Conclusion：(1)There were some differences of similarity, species and distribution diversity inintestinal microbial communities of four abnormal hilit hyperpietics and normal hilit group.(2)The phylogenetic trees of intestinal flora diversity of four abnormal hilit hyperpietics andnormal hilit group in Uighur nationality and the phylogenetic trees show that the existing ofFirmicutes, Bacteroidetes, Verrucomicrobia, Butyrate-producing bacteriumand and Unculturedbacterium were some factors that can be determinant for the form and development ofabnormal hilit hypertension.