Study On Chemical Constituents Of The Aerial Parts Of Scutellaira Baicalensis Georgi

The dry aerial parts of scutellaria baicalensis Georgi were extracted with ethanol, theconcentrated alcohol extractwater was dispersed with water, extracted with petroleum ether,ethyl acetate and n-butanol respectively, petroleum ether extractive obtained accounted for1.9%of the quality of the aerial parts of scutellaria baicalensis Georgi, ethyl acetate extractiveaccounted for0.3%and the n-butanol extractive accounted for1.2%.By using of the methods column chromatography, HPLC separation and recrystallization,petroleum ether extract, ethyl acetate extract and n-butanol extract were isolated, purified,obtained21single compounds. Through the physical, chemical properties and spectral dataanalysis, their structures were identified: methoxyphaeophorbide (1),β-sitosterol-3-O-β-D-glucoside (2), p-hydroxybenethyl ethanol hexadecanoic methyl ester (3),α-apinasterol (4), β-sitosterol (5), glyceride-1-linoleic-3-linolenic acid ester (6),5,6,7,4′-tetrahydroxyflavanone (7), ethoxyphaeophorbide (8), n-octadecanol (9),5,7-dihydroxy-6-methoxyflavanone (10), oroxylin (11), chrysin (12), apigenin (13), Lutein(14),5,4′-dihydroxy-6,7,8,3′-tetramethoxyflavone (15),5,2′-dihydroxy-6,7,8,3′-tetramethoxyflavone (16),5,2′-dihydroxy-7,8,6′-trimethoxyflavone(17),5,2′-dihydroxy-7,8-dimethoxyflavone (18),5,2′-dihydroxy-6,7,8-trimethoxyflavone (19),apigenin4’-β-D-glucopyranoside (20), apigenin-7-β-D-glucopyranoside (21). Among thesecompounmds, compound3is a new and have not reported,7,10,11, and15~21are the firsttime have been isolated from the aerial parts of scutellaria baicalensis Georgi.The petroleum ether, ethyl acetate and n-butanol extract with the aerial parts ofscutellaria baicalensis Georgi were tested with anti-plant pathogens activity, and the resultsshowed that: The ethyl acetate extract has inhibitory action for Tomatoes downy mildew virus,Cucumber angular spot germs and Rhizoctonia solani, and enhanced with increasingconcentrations.