Gualou Xiebai Banxia Decoction Of Myocardial Ischemia Reperfusion Injury JAK-STAT Cell Signaling Pathways Adjusting Research

Objective:Explore GualouXiebaiBanxia Decoction of myocardial ischemia reperfusion injury JAK-STAT Cell signaling pathways adjusting research.Methods:60male SD rats randomly divided into blank control (A) group, ischemia reperfusion (B) group (I/R), ischemia preconditioning (C) group (IPC), GXBD pretreatment (D) group, AG490processing (E) groups, and only8(victim eliminate). Ligation rat the left anterior descending coronary artery30minutes,90minutes reperfusion making myocardial ischemia reperfusion injury, igl times every day, model for3consecutive days, the end time ig1h line after coronary artery ligation. A group of group B and capacity to physiological saline, group D group E with5.58g kg-1doses ig to medicine, AG490group E with2mg kg-1dose, to build mode1h before sublingual intravenous,(1) during the observation group rat electrocardiogram ST segment changes (2) made after the end of each model, observe the rat serum lactic acid dehydrogenation (LDH) changes (3) each myocardial infarction area of change (4) each observation rat pathological morphology change each (5) determination rat adenosine (Ado) gentle shock peptide (BK) level (6) observation each rat heart muscle tissue, the JAK2STAT3protein expressionResults:(1) GuaLou Xie sprouting white soup pretreatment and against myocardial ischemia preconditioning ischemia reperfusion injury rat electrocardiogram st-segment elevation (2) model group and AG490group serum level of LDH significantly increased (p<0.05), Ado and BK level decreased significantly (p<0.05), myocardial infarction area expanded significantly (p<0.01), HE after dyeing cardiac cells present typical pathological changes, STAT3JAK2, protein expression significantly reduce (p<0.05)(3) group, GXBD pretreatment IPC group were significantly reduced serum LDH (p<0.05), Ado and BK level significantly increased (p<0.05), myocardial infarction narrow area of significant (p<0.01), HE after dyeing myocardial cell damage degree significantly reduce JAK2, STAT3protein expression, significantly increased (p <0.05)Conclusion:GXBD can through the JAK-STAT cell signaling pathways, improve the mediated electrocardiogram st-segment level and change the myocardial cell pathological morphology of arrhythmia, reduce the incidence, LDH content and myocardial infarction area, STAT3JAK2, raising the protein expression, rise, BK、Ado content, to protection to ischemia reperfusion myocardial...