The Effects Of Buyanghuanwu Decoction On The Signal Transduction Of Nf-κB Factorn And Cell Proliferation In Rats After Focal Cerebral Ischemia Injury

Objective:To explore The effects of BuyangHuanwu Decoction on the signal transduction of NF-κB factorn and cell proliferation on rats following focal cerebral ischemia to provide proof for clinic to facilitate work.Methods:Rat model of cerebral ischemia injury was created by the middle cerebral artery occlusion (MCAO) with suture method, healthy adult SD rats were randomly divided into the normal group,Sham-operated group,ischemia group and BYHWD treatment group,MC treatment group,PDTC treatment group, Animals were receiveid different processing and killed at7,14,21Day point,after operation.The nervous deficit symptoms were observed and the infarct size ratio was measured by triphenyltetrazolium chloride (TTC) staining after the cerebral ischemia. In addition, The expression of NF-KB/p65, NF-κB/p50, I-κB and BrdU were detected by immunohistochemistry technology. Result:1.Neurological function deficit symptom scores and infarct size ratio: compared with the normal group and the sham group,The model group had seriously neurological deficit and increase the cerebral infarct size ratio(P<0.01),the cerebral infarct size ratio and,neurological function deficit symptom scores decreased along the survival time increasing at7,14day point..after7,14,21days’treatment, the treatment groups all could significantly improve the grade of nerve function in comparison with the model group(P<0.05or0.01), and significantly decrease the cerebral infarct size ratio(P<0.01). the difference Between the treatment groups is not significant (P>0.05). the cerebral infarct size ratio decreased along the survival time increasing.2.The result of the expression of NF-KB/p65, NF-κB/p50by immunohistochemical stainingshow that:the normal and Sham group have a few positive NF-κB/p65, NF-κB/p50cells;In model group, the expression of NF-κB/p65, NF-κB/p50seriously increased(P<0.01), The expression of NF-κB/p65, NF-κB/p50decreased along the survival time increasing,BYHWD, MC, PDTC,treatment group, all could significantly inhibit the expression of NF-κB/p65, NF-KB/p50(P<0.05or0.01). after7,14days’treatment, the difference Between the treatment group is not significant.after21days’treatment BYHWD displays the trifle superiority in comparison with PDTC(P<0.05). 3.The result of the expression of I-κB by immunohistochemical staining show that the normal and Sham group have an massive positive I-κB; In model group, the expression of I-κB seriously decreased BYHWD, MC, PDTC, treatment group, all could significantly increase the expression of I-κB(P<0.01), after7days’treatment,MC treatment result is better than the other treatments result, after14days’treatment(P<0.01), BYHWD,MC treatment result is both better than PDTC treatment(P<0.01), after21days’treatment, BYHWD treatment result is better than MC and PDTC treatment(P<0.01), at14,21days’point the expression of I-κB at BYHWD,MC treatment group decreased along the survival time increasing.4.The result of the expression of BrdU positive cellby immunohistochemical staining show that the normal and Sham group have a few BrdU positive cell, In model group, the expression of BrdU positive cell increase,but not much, The expression increased along the survival time increasing, BYHWD, MC, PDTC, treatment group, all could significantly increase the expression of BrdU positive cell(P<0.05or0.01), after7,14days’treatment, the difference Between the treatment group is not significant (P>0.05), after21days’treatment BYHWD displays superiority in comparison with the other the treatment groups (P<0.01). Conclusion:1. BYHWD has the same efficacy as MC,PDTC on deereasing infarct size ratio and neurological function deficit symptom.2. BYHWD has significant protection on cerebral ischemia injury in rats, BYHWD has the same efficacy as MC,PDTC on control inflammatory factors expression in Protein,which may be related to the inhibition of inflammatory cytokine secretion and expression.and inducing the inflammation in brain tissue.The result maybe show that BYHWD protect brain by regulate the the expression of I-κB to control signal transduction of NF-κB factor,revealed the mechanism of inflammatory action in the view of signal transduction of NF-κB factor.3. BYHWD has the same efficacy as MC, PDTC on tissue self-repair by promote cell proliferation after focal cerebral ischemia, cell proliferation’s tendency and factor of signal transduction of NF-κB change tendency prompt that BYHWD possibly affects the cell proliferation through regulating signal transduction of NF-κB.4. the function of BYHWD to be more remarkable at the recovery period.