Protective Effects And Mechanisms Of Caowei-BuYangHuanWu Against Neuronal Injury In MACO Rat

Foreword: Ischemia stroke is a frequent and common disease with high mortality and high disability in clinical work. The study show that neuronal injury following cerebral ischemia is the main part of pathological mechanism's study. So the searching of drugs for protect cerebral ischemia neuronal injury is a hotspot in this domain. BuYangHuanWu is one of the medicament with positive curative effect and obvious trait for Ischemia stroke.Objective:(1) Investigate the trait of the ChaoWei-BuYangHuanWu by contrasting the main effective element in ChaoWei-BuYangHuanWu and BuYangHuanWu, make some rewarding work for the medicament reform of Chinese traditional medicine.(2) Study the effection of protecting the neuronal injury following cerebral ischemia , and make sure the mechanism by the experimentation in MCAO rat. Offering some experience for the clinical work on Ischemia stroke.Methods:(1) Make sure the effective element of ChaoWei-BuYangHuanWu by series test: Study the essential element of Chinese angelica Root, Red Peony Bark, Chuangxiong Rhizome, Safflower and Peach Kernal by TLC; and measure the content of the main useful element in ChaoWei-BuYangHuanWu; Make sure the content of specifically metal , complex and water-solubility- lixivium \alc-ohol-solubility-lixivium by the ways which was recorded by PharMCAOpoeia Of People's Republic Of China.(2)Make MCAO rats by special ways, divide the rats to 6 group; and abserve the rats at 1\ 4\ 7 and 14 days, observe and record the given symptoms, give the grade by the symptoms; after the test, kill the rats and take blood , then detect the actives of SOD and MDA in blood by given ways ; after all, take the brain , Immunohistochemical avidin-biotin peroxidase complex method was applied to detect the expression of NGF and his Receptor TRKA; And In Situ Hybridization with Nick-Translated Probes was applied to detect the PI3K/PKB, which was the main Intracellular signal transduction of NGF.RESULTS:(1) showing the same color spot in the corresponding place on the chromato-gram of each sample of the two groups measured by TLC, and the spot is more distinctive in the effective element of ChaoWei-BuYangHuanWu.It is showed that the content of Astragaloside IV in ChaoWei-BuYangHuanWu is equivalent in the traditional decoction, the content of arsenide and specifically metal is not in superscale. It is found that the water-solubility- lixivium is 1.22 times and the alcohol-solubility-lixivium is 1.26 times in ChaoWei-BuYangHuanWu than in the traditional decoction.(2) ChaoWei-BuYangHuanWu can reduce the grade of the the neuronal injury following cerebral ischemia, improve the pathological injury of cerebral ischemia. It is statistically different than the model group and artificial operation group, the effection of high dosage ChaoWei-BuYangHuanWu is statisti- cally better than traditional decoction.(3 ) ChaoWei-BuYangHuanWu can improve the active function of SOD, reduce the active function of MDA. This function is statistically different than the model group and artificial operation group, the effection of high dosage ChaoWei-BuYangHuanWu is statistically better than traditional decoction.(4) ChaoWei-BuYangHuanWu can raise the expression of NGF and his Receptor TRKA after cerebral ischemia, and boost up the signal transduction of PI3K/AKT.This function is statistically different than the model group and artificial operation group, the effection of high dosage ChaoWei-BuYangHuan Wu is statistically better than traditional decoction.CONCLUSION:(1) BuYangHuanWu decoction and it's ChaoWei-preparation can improve the neuronal function grades and pathological changes of rats with partial cerebral ischemia;It can improve the active function of SOD, reduce the active function of MDA to weaken the toxic of superoxide free radicals. It is protective to the injuried neurocyte of cerebral ischemia.(2) ChaoWei-BuYangHuanWu kept the effctive element, as well as the traditional decoction and it is better than the traditional decoction in solubility -lixivium.(3) ChaoWei-BuYangHuanWu can boost up the signal transduction of PI3K/ PKB that is a kind of Neurotrophic factor. It also can raise the expression of NGF and his Receptor TRKA after cerebral ischemia. This is likely to be one of the protective mechanism of ChaoWei-BuYangHuanWu to neuronal injury following cerebral ischemia.(4) 1/2 equivalent effective dosage of ChaoWei-BuYangHuanWu can make the same effecion of the traditional decoction. It is prone to be effective stronger when used the equivalent effective dosage.Above all, this topic adopted Pharmaceutical method, method of pharMCAOlogy, Immunohistochemical method and molecular organism, demonstrated the asc- endancy of promoting solubility-lixivium of effective element in traditional Chinese medicine (BuYangHuanWu decoction) by contrasting ChaoWei-BuY- angHuanWu in which ultramicro technology was used and traditional BuYang- HuanWu decoction, and revealed the mechanism of action in the view of signal transduction of Neurotrophic factor. So this topic had great new ideas.