Analysis Of Differentially Expressed Proteins In Sera Of Coronary Artery Disease

PART ONE Differentially expressed proteins analysis insera of stable angina pectoris patientsObjective: To explore the differentially expressed proteins in sera ofstable angina pectoris patients.Method: Serum samples of stable angina pectoris patients (n=50) andhealthy controls (n=50) were respectively separated by two-dimensional gelelectrophoresis and MALDI-TOF-MS to define the differentially expressedproteins.Result: Inter-alpha-trypsin inhibitor family heavy chain-related protein(IHRP) and zinc finger protein A20expressed in sera of stable anginapectoris patients, but none in healthy controls.Conclusion: The differentially expressed proteins between stableangina pectoris patients and healthy controls may play an important role inpathomechanism of stable angina pectoris. PART TWO Peptide finger printing analysis in sera of AcuteMyocardial Infarction patientsObjective: To analyze peptide finger printing in sera of acutemyocardial infarction patients.Method: Serum samples of acute myocardial infarction patients (n=50)and healthy controls (n=50) were respectively separated T bytwo-dimensional gel electrophoresis and MALDI-TOF-MS to define thedifferentially expressed proteins and analyze peptide finger printing in seraof acute myocardial infarction patients.Result: Inter-alpha-trypsin inhibitor family heavy chain-related protein(IHRP) was found in sera of acute myocardial infarction patients, but nonein controls. ApolipoproteinA-I and serotransferrin were considerablydecreased, whereas human complement component C3and haptoglobinwere considerably increased in sera of acute myocardial infarction patientscompared to healthy controls.Conclusions: These five proteins may be involved in the pathogenesisof acute myocardial infarction. They may provide candidate biomarkers forthe diagnosis and therapy of acute myocardial infarction.