Determination Of Erythrocyte CR1in Children With Obstructive Sleep Apnea Hypopnea Syndrome

ObjectiveWith the development of medical technology, obstructive sleep apnea hypopnea syndrome (OSAHS) in children is greatly improved.To study the changes and clinical significance of erythrocytic immune function in children with OSAHS, by detecting the level of erythrocyte complement receptor type1(CR1)Methods30cases of OSAHS and30cases of healthy controls were recruited. The level of erythrocyte CR1was assayed by enzyme linked immunosorbent assay(ELISA). All subjects except for immune system disorders and allergic diseases, no history of pharyngeal surgery and cognitive dysfunction in the first four weeks of the monitoring of upper respiratory tract infection, two groups of age, sex and general characteristics of the difference was not significant (P>0.05). The methods of ELISA referenced Guo Feng’s. CR1were pre-coated in ready to use96-well strip plate, prepared7wells for standard,1well for blank, addeded100μl the standards and test samples to each well incubated2hours at37℃after covering it with the plate sealer, added100μl biotin-conjugated antibody preparation specific for CR1, incubated1hours at37℃, washed3times,the unbound biotinylated antibody was washed off, and then added100μl avidin conjugated to Horseradish Peroxidase(HRP), incubated30min at37℃, washed5times with wash buffer, Added90μl TMB substrate solution to each well, pretected it from light, incubated5～10min at37℃, then added50μl stop solution, read at the450nm immediately from the micro plate reader. The kit shown in standard drawing a standard curve, the corresponding concentration compared to the standard curve, multiplied by the corresponding dilution factor, that is to come to the actual concentration of the sample.CR1activity was detected by the erythrocyte C3b receptor rosette (ECRR)and erythrocyte immune complex rosette(EICR). The test samples of two groups were washed4times with PBS, dubbed1.25×10(?)/ml. take the two test tubes, added100ul samples to each tube, added C3b sensitized zymosan working solution100ul to one tube, added the zymosan test solution100ul to the other, incubated30min at37℃, added0.9%NS100ul, added0.25%glutaraldehyde50ul, dried, added methanol and Rui’s liquid staining10seconds. High power microscope, counting a red blood cell adsorbed two or more than two yeast as a wreath. Count of200red blood cells, calculate the percentage.SPSS13.0software was used for statistical processing and analysis, measurement data were indicated by x±s,"t test" was used for statistical analysis. P<0.05was considered that difference was statistically significant.ResultsThe expression of CR1was obviously lower than those in control group (P<0.05), It was compared to show that EICR in children with OSAHS was higher than that in the control group, ECRR was lower than that in the control group and the erythrocyte complement receptor type1was related to apnea hypopnea index(AHI) negatively but to lowest oxygen saturation(LSaO2) positively.ConclusionsThe erythrocytic immune function in children with OSAHS was reduced and related with AHI and LSaO2.This may affect the systemic immune function.