| Successful root canal therapy is influenced by the root canal preparation, while the good root canal preparation needs appropriate root canal irrigation. At present there are a lot of root canal irrigations in the clinical application, but no one can achieve the four important elements which are needed. Based on the traditional Chinese herbal medicine advantages and characteristics, we mix16%Gallnut and Scetullaria extraction up to attain a new root canal irrigation. From the extracted teeth experiment in vitro, we can evaluate the efficiency of gallnut and scetullaria water extract as a root canal irrigation by comparing the differences of16%Gallnut and Scetullaria extraction and common clinical root canal irrigation in bacteriostasis, root canal cleaning ability and apical sealing, which provide the experimental basis for clinical application.Objective:1. To observe the antibacterial activity of different root canal irrigations on Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn), we flush the root canal with each of them. In this way, we can evaluate the antibacterial activity of16%Gallnut and Scetullaria extraction as a root canal irrigation.2. To observe the activity of different root canal irrigations in removing smear layer,we flush the root canal with each of them. In this way, we can evaluate the activity of16%Gallnut and Scetullaria extraction in removing smear layer as a root canal irrigation.3. To observe the the microleakage after irrigating, we flush the root canal with different root canal irrigations. In this way, we can evaluate the influence of16%Gallnut and Scetullaria extraction on the apical sealing as a root canal irrigation.Methods:1.90extracted teeth meeting the requirements were chosen. Conventional open-pulp, removing the pulp and preparing the canals by using ProTaber/crown-down technique, and then establish the Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn) teeth infection model. These teeth were divided randomly into10groups with each group9teeth:experimental groups A-Pg and A-Fn were irrigated by16%Gallnut and Scetullaria extraction, while control groups were B-Pg、B-Fn、C-Pg、C-Fn、D-Pg、D-Fn、E-Pg、E-Fn (0.5%NaClO、0.5%NaClO、17%EDTA、17%EDTA、3%H2O2、3%H2O2、0.9%NaCL、0.9%NaCL). The number of bacteria were measured in the teeth infection model root canal before and after irrigation.2.60extracted teeth meeting the requirements were chosen. These teeth were divided randomly into5groups with each group12teeth:experimental groups A:16%Gallnut and Scetullaria extraction, control groups B、C、D、E (0.5%NaClO、17%EDTA、3%H2O2、0.9%NaCL). Conventional open-pulp, removing the pulp and measuring the length of the root canal. According to the requirements of the experimental groups, the root canals were prepared by using ProTaber/crown-down technique, and separately irrigated by10ml of16%Gallnut and Scetullaria extraction、0.5%NaClO、17%EDTA、3%H2O2、0.9%NaCL. The teeth were split longitudinally, dehydrated, sprayed gold, and observed with SEM to evaluate the smear layer.3.50extracted teeth meeting the requirements were chosen. These teeth were divided randomly into5groups with each group10teeth:experimental group A:16%Gallnut and Scetullaria extraction, control groups B、C、D、E (0.5%NaClO、 17%EDTA、3%H2O2、0.9%NaCL). Conventional open-pulp, removing the pulp and measuring the length of the root canal. According to the requirements of the experimental groups, the root canals were prepared by using ProTaber/crown-down technique, and separately irrigated by10ml of16%Gallnut and Scetullaria extraction0.5%NaClO、17%EDTA、3%H2O2、0.9%NaCL. Leakage along the root filling was measured by concentration of glucose in apical reservoit at thirty days with O-TB method.Results:1. The Porphyromonas gingivalis (Pg) groups:the numbers of bacteria in each root canal before irrigation were no statistically difference(P>0.05); the numbers were effectively reduced after irrigation in each group and were statistically more effective than that before irrigation (P<0.05); A-Pg colonies numeration difference was less than B-Pg and there was statistically difference (P<0.05); A-Pg colonies numeration difference was higher than C-Pg、D-Pg、E-Pg and there was no statistically difference between A-Pg and C-Pg、D-Pg(P>0.05), but there was statistically difference between A-Pg and E-Pg(P<0.05). The Fusobacterium nucleatum (Fn) groups:the numbers of bacteria in each root canal before irrigation were no statistically difference(.P>0.05); the numbers were effectively reduced after irrigation in each group and was statistically more effective than that before irrigation (P<0.05); A-Fn colonies numeration difference was higher than C-F、D-Fn、E-Fn and there was no statistically difference between A-Pg and C-Fn(P>0.05), but there was statistically difference between A-Fn and D-Fn、E-Fn(P<0.05).2. There was no statistically difference between the upper root1/3and middle root1/3part of the dentin debris in experimental group(P>0.05), but the difference between the upper root1/3and the apical1/3part of the dentin debris was significant(P<0.05), the middle root1/3and the apical1/3part was no statistically difference(P>0.05).In the upper root1/3, the middle root1/3and the apical1/3part, the dentin debris between the group A and group B, group C, there was no statistically difference(P>0.05), but the dentin debris in group D and group E was more, and there was statistically difference(P<0.05). 3. Compared group A and groupB、C respectively, although concentration of glucose higher than the two groups, but the difference between group A and groups B、C was no significant(P>0.05). Compared group A and group D、E respectively, although concentration of glucose higher than the two groups, but there was statistical difference between the two groups(P<0.05).Conclusion:1. The study showed that the antibacterial activity of16%Gallnut and Scetullaria extraction as a root canal irrigation was lower than0.5%NaC10, but higher than17%EDTA、3%H2O2、0.9%NaCL in the same conditions.2. The study showed that the ability of16%Gallnut and Scetullaria extraction as a root canal irrigation in removing smear layer and0.5%NaClO、17%EDTA was no statistically difference, but higher than3%H2O2、0.9%NaCL in the same conditions.3. The study showed that the microleakage after root sealing of16%Gallnut and Scetullaria extraction as a root canal irrigation and0.5%NaClO、17%EDTA was no statistically difference, but lower than3%H2O2、0.9%NaCL, no effect on root canal sealing.To sum up, compared to common clinical root canal irrigation,16%Gallnut and Scetullaria extraction had better ability on antibacterial activity, root canal cleaning and apical sealing. |
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