The Study On The Pharmacy And Pharmacodynamics Of Zhike Pingchuan Patch

Objective:①In our work, we will establish the quality standard and study on the stability of Zhike Pingchuan Patch.②Through the study of pharmacodynamics and toxicology to investigate the therapeutic action on the disease of respiratory system and security of Zhike Pingchuan Patch.Method:①To establish the quality standard and preparation technology of Zhike Pingchuan Patch, we will test the appearance, relative density, difference of filling weight and hygienics. Using TLC identification method, we can identify the ingredient of Cassia twig, Storax, Cortex Moutan Radicis and Pericarpium Citri Reticulatae. Using sample observation method in room temperature test, we can know the preliminary stability of Zhike Pingchuan Patch.②Through the mice cough induced by ammonia water test, phenol red method, mouse ear swelling method, guinea pig histamine induced asthma acetylcholine chloride phosphoric acid method, disc diffusion method, we test the cough incubation period and times in five minutes, the content of phenol red in tracheal lavage fluid, swelling extent of ear, guinea pig asthma with a latency period and the size of inhibition rings in vitro in order to investigate the therapeutic action on the disease of respiratory system.③By the use of the method of acute toxicity test, the diet and activity situation were observed. And anatomical observation of organs to determined if there exist death and pathological change after using Zhike Pingchuan Patch.④After using Zhike Pingchuan Patch on guineas that the skin of guineas were damaged, the skin allergic reactions and skin toxicity were observed.Result:①Preparation process includes:There were16kinds of TCM in Zhike Pingchuan Patch. Except for storax, frankincense and myrrh, the other13kinds of TCM were decocted with four times volume60%ethanol for two times and one hour each time and the merge the fluid. The fluid was concentrated to less than800ml. Then powder of storax, frankincense and myrrh was put into the fluid and decocted with three times volume ethanol for 30minutes. At last laurocapram, carmowax, propanediol, propanetriol and ethylparaben were put into the concentrated solution. After1000ml fluid stewing for12hours, the supernatant fluid was split charging.②Quality standard:The preparation of Zhike Pingchuan Patch is pale brown liquid and slightly sweet. The value of relative density was regulated in the range of1.03-1.05g/ml. There was no more than1000bacterial and no more than100mildew in lml Zhike Pingchuan Patch liquid medicine. And there was no colon bacillus, salmonella and live acarid.③The stability test shows that all kinds of indexes were no significant change of product.④The pharmacodynamic experiment shows that there were antitussive, expectorant, anti-asthma, analgesic, anti-inflammatory and antibacterial effect in various degree with different dosage of Zhike Pingchuan Patch.⑤The maximal dosage of Zhike Pingchuan Patch stock solution and concentrated solution were32g/kg and166g/kg respectively. And the dosage was equal to clinical dosage280times and1456times. There was no death and there was no pathological change after anatomical observation.⑥There was no skin allergic reactions and skin toxicity on guineas after useing Zhike Pingchuan Patch.Conclusion:①The preparation of Zhike Pingchuan Patch is correspond with national standards. The quality is steady and the retention period is24months.②Zhike Pingchuan Patch could improve the respiratory disease of experimental animal.③Clinical use of Zhike Pingchuan Patch is quite safe.