| Objective:To study the neuroprotective effect of Extract of Acanthopanax senticosus Harms(EAS) against MPTP-induced mice model of Parkinson’s disease and its mechanism.Methods:1.The Parkinson’s disease mice model was induced by1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Hydrochloride (MPTP-HCl,30mg/kg daily for5days).2. High dose group and low dose group were medicated with EAS for20days, dose amounted to182mg/kg and45.5mg/kg daily respectively.3. The behavioral testing of mice was assessed using pole-climbing test.4. The levels of Dopamine (DA) and Homovanillic Acid (HVA) in striatum were determined by Ultra-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC-ToF-MS).5. The levels of dopamine receptor land2in striatum were assayed simultaneously with the help of immunohistochemical method.6. The level of Caspase-3protein in substantia nigra was analyzed by Western Blot.Results:1. From Day5during the administration of EAS, pole-climbing time in low and high dose group were significantly less than model group (P<0.05).2. Compared with model group, the DA level of striatum in low-dose group was significantly higher (P<0.01), the number of dopamine receptor1and dopamine receptor2-positive cells in low and high dose group were significantly less(P<0.05), the Caspase-3protein level of substantia nigra in low and high dose group were significantly less(P<0.05).Conclusions:1. Behavioral testing shown that EAS have some improving effects on pole-climbing behavior in Parkinson’s disease mice model, and strengthen their movement coordinated ability.2. EAS can increase the DA level of striatum in PD mice, this show that EAS have some protective effects on DA nerve cell.3. EAS can inhibit DRD1and DRD2’s up-regulation in PD mice, and they can balance the functions of direct pathway and indirect pathway in basal ganglia.4. EAS can can inhibit Caspase-3activation, this show that EAS have some protective effects on DA nerve cell indirectly. |
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