A Study Of Wound Healing On Topical External Cell Growth Factors Atfer Fractional Carbon Dioxide Laser Therapy

The pursuit of beauty is getting higher and higher with the continuousimprovement of living standards, chronic skin aging, wrinkles, acne scars, surgical scarsand other skin diseases such loss-capacitive have been growing attention extensively.From the traditional dermabrasion to plastic surgery, to minimally invasive surgery,various treatment methods continue to be applied clinically. However, the inadequacy ofthese technologies is likely to cause postoperative erythema, edema and pigmentation,thereby prolonging the patient’s downtime. In recent years, the Fractional UltrapulsedCO2laser provide increased efficacy in the treatment of this skin diseases, reducedpostoperative adverse events significantly, and reduced the downtime. However, thisnew technology is not without trauma, and the treatment will lead to a certain degree ofacute thermal damage, and wound healing will take some time, thus affecting thedowntime. How to reduce the adverse reaction and shorten the patients’ downtime afterFractional Ultrapulsed CO2laser are becoming the hot issues. As we all know, anywound healing is a complex process by many cytokines involved in the body. Tissuegrowth factor concentration is limited under normal circumstances, and adding externalcell growth factors can accelerate wound healing significantly. Currently, the moststudied external cell growth factor is epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF).This study was to explore the efficacy of the additional topical application ofrecombinant human epidermal growth factor (rhEGF) and recombinant bovine basicfibroblast growth factor (rb-bFGF) after Fractional Ultrapulsed CO2laser therapy.ObjectiveThe purpose of this study was to compare the wound healing efficacy of theadditional topical application of recombinant human epidermal growth factor (rhEGF)and recombinant bovine basic fibroblast growth factor (rb-bFGF) after FractionalUltrapulsed CO2laser therapy. In order to provide a theoretical basis for clinical skincare, shorten downtime and reduce adverse reactions after laser therapy.MethodsThe back skin of twenty SPF healthy male guinea pigs were divided into fourregions of I, II, III, IV after hair removal, then measure the skin elasticity and melanincontent index (MCI) of four regions with a skin tester of physiological functions. Thenthese four regions were irradiated respectively with Fractional Ultrapulsed CO2laser,and four regions of I, II, III, IV respectively external rhEGF、rb-bFGF、rhEGF jointrb-bFGF (combination therapy group) and physiological sodium chloride solution(control group). The general condition of the wound healing was observed grossly. Atday7,14and28after laser therapy measure the skin elasticity and MCI of four regionsagain, and four guinea pigs were killed randomly at three different time points to do thepathological examination and compare the change of skin flexibility and fibroblastsnumber. ResultsThe scab of therapeutic groups falling off completely after carbon dioxidefractional laser therapy at3~7d, the scab of control group didn’t fall off completely;The wounds of four groups were healed completely at14~28d, and the tissues aretender and red in laser-treated areas compared with the surrounding area is notlaser-irradiated. Comparison among the four groups: the wound area of treatmentgroups is smaller and the color is redder than the control group. The skin elasticity ofcombined treatment group were higher than other groups at day28after laser treatment,the index of combined treatment group were262.29±62.40，the rhEGF treatment groupwere202.00±65.62，the rb-bFGF treatment group were188.86±35.02and the controlgroup were167.14±42.49，there was apparent statistically significant. Although theMCI of four groups compared with there was no statistically significant at day28, theMCI of three treatment groups were lower than the MCI of before laser treatment, andthe control group’s was higher than before laser treatment. The results of pathologicalexamination showed that the arranged of collagen fibers of combination therapy groupwas ordered and compact, and then the control group was disorder and loosened.Conclusion:External cell growth factors after Fractional Ultrapulsed CO2laser could speed upwound healing, improve skin elasticity, reduce the MCI, compact the collagen and noadverse reactions, so it’s worth to clinical application. Combined use cell growth factorcould reach the best available treatment.