The Intervention Of Sesamin On Human Lung Cancer Cell Lines A-549

Objective:To investigate the intervention of sesamin on antiproliferation and apoptosis induction of human lung adenocarcinoma cell line A-549in vitro, provide a theory basis for traditional medicine treatment in antineoplastic therapy.Methods:Cultivate human lung adenocarcinoma cell line A-549in vitro.Measure the antiproliferation and cytotoxicity of sesamin on human lung adenocarcinoma cell line A-549by MTT; Morphological changes were observed by inverted microscope and HE staining; Flow cytometry was used to investigate the apoptosis and the cell cyele distribution and the expression of Bcl-2and Bax; The apoptosis rate was determined by AnnexinV/PI staining; The expression of apoptosis protein kinase such as Caspase-3、Caspase-8、Caspase-9around medicine treatment Were detected by immunohistoche-mistry.Results:(1)MTT assay showed that sesamin have significant inhibition in the growth of A-549cell line and the effect depend on concentration and time.(2) Observed by inverted microscope showed the volume of the cell A-549decreased and get round, nuclear condensed chromatin, the junction between cell loosed, stick ability decreased, HE staining showed a typical cell morphology changes such as the cytoplasm dehydrated and concentrated and enhanced Haematoxylin-eosin staining;(3)Flow eytometry showed a cell apoptosis peak in48h after40μg/ml medicine treatment, cell increased in S phase and decreased in Go/G1Phase, most cell stay in S phase. The expression of Bcl-2deereased, while the expression of Bax increased.(4) Immune cells chemical showed that the expression of Caspase-3、8、9increased, it has a significantly different compared with control group(P<0.05).Conclusions:1. Sesamin could signifieantly inhibit the growth of A-549cells and the effect was in a dose-dependent and time-dependent manner.2. Sesamin could arrest the A-549cells in the S phase.3. Sesamin tinduced apopsis via two main pathways:the mitochondrial pathway and the death receptor-mediated pathway.4. Sesamin has a significant effect in anti-tumor, It would hopefully exploit as a new anti-tumor or Supplementary medicine...