| At present, interventional treatment has become the main treatment of coronary atherosclerosis, which percutaneous transluminal coronary angioplasty has become the most mature, but also the most widely used means. Higher stenosis rate limit long-term efficacy, immediately after the joint intervention with a variety of drugs and methods, its narrow rate is still about20%. From surgical intervention is the first time in the past two decades, clinicians and scholars have been trying to find countermeasures to solve the problem of restenosis, but also made considerable progress, but until now, restenosis after interventional treatment the most important complications. At the same time, medicine believes that qi deficiency and blood stasis and phlegm is restenosis of the main reason of this vacuity restenosis characteristics of percutaneous coronary intervention to clear the blood rule "standard", but the organ virtual loss "this still exists, therefore, we start from the the restenosis TCM pathogenesis According to Qi, activating blood circulation, removing blood stasis, phlegm, Astragalus, Codonopsis, safflower, Chinese angelica, Sichuan dome is selected, three edge, Puhuang,, Yan Hu, digital components of Chinese medicine Tongmai hoist side.Purpose Early studies have confirmed that Astragalus, Codonopsis, safflower, Chinese angelica, Sichuan dome, triangular, Puhuang, Yan Hu such as promoting blood circulation laboratory to be adopted by the grouping experiment for modeling high-fat feeding of the animals in elucidating the tumor necrosisunder the premise of the factor, interleukin1,6, pathogenesis, select the above indicators as a reference, the production of vascular HE sections before and after comparison, to further verify the mechanism and efficacy of Tongmai hoist side treatment of restenosis.MethodSelected60New Zealand white rabbits were divided into groups, control group, traditional Chinese medicine, the low concentration group (n=12), basic feeding of the blank group, the remaining high-fat feeding, basal diet plus5g/(kg. d) fat and0.5g/(kg. d) cholesterol feeding feeding January surgery model, methods for re-anesthesia state from the rabbit carotid artery retrograde into the balloon catheter to the coronary Department, followed by expansion of the ballcapsule and stretch3times. Began to drench the week before surgery, drug fried in the Experimental Animal Center of Wuhan First Hospital, drug concentrations1.5g/ml,5.6ml/(kg. d) The medicine of the high concentration of traditional Chinese medicine group, the concentration of group fed with2.8ml/(kg. d) traditional Chinese medicine, the low concentration group were fed1.4ml/(kg. d) The traditional Chinese medicine, the control group, model group fed with10ml saline. The two batches were sacrificed after30days and60days, take the rabbit serum, centrifuged, and then using ELISA test endothelin, tumor necrosis factor, interleukin1,6, the four indicators. And took the damaged blood vessels and around1.5cm at rapid immersion in formalin solution, underwent paraffin sections.ResultIn this study, endothelin, interleukin-1,6, tumor necrosis factor as a serological test indicators reflect inflammation and vascular intimal thickening with a certain representative from the results, the model group, high fat diet with normalcompared with a significant difference (p<0.05), indicating by the high fat diet, rabbit hyperlipidemia, the index significantly increased the risk of coronary heart disease increases. Low concentration of the drug high school group and model group were significant differences (P <0.05), traditional Chinese medicine group drench, reducing the risk of restenosis of coronary heart disease. Drug high, medium and low concentration groups compared with the best in the concentration of group efficacy, and the high and low concentration group was statistically significant, indicating that the drug concentration has a certain influence on the efficacy. Moderate concentrations of drug efficacy.30days60days between the groups no significant differences in contrast. Vascular HE sections visible balloon injury zone relative to the blood vessels of other parts of the intima is thicker, indicating that the model was successful. At the same time, the intima-media thickness of the three drug groups compared with model group. Can prove that the intervention effect of drugs on restenosis. discussThi s experiment after30days after the first four weeks of model ing and modeling to the60-day high-fat feeding, rabbits appeared obesity, hyperlipidemia, hyperinsulinemia, under the microscope visible intimal thickening. RS model specimens from animals early formation of the artery wall in addition to Asia thrombosis [8]:(1) within the first month after surgery, inflammation lesions more common in lesions seen a large number of tissue cells, lymphocytes, neutrophils and granulation organizations.(2) the degree of inflammation and arterial positively related to the degree of damage.(3) neointimal thickness is positively correlated with the degree of injury.(4) neointimal thickness is positively correlated with the degree of inflammatory reaction.(5) a greater degree of injury and inflammation together lead to intimal thickening, and the thicker intimal hyperplasia, neointimal area is larger, the higher the RS rate. HE staining of the blood vessels visible lesion of the model group, intimal thickness was significantly greater than non-adjacent parts of the lesion, indicating that surgery on the more obvious effect of rabbit vascular intimal injury. Intimal hyperplasia of the drug group was lower than the model group, and the lowest concentrations of endometrial hyperplasia, showing the inhibitory effect of drugs on the inflammatory response. From the30days and60days two groups of model point of view, continued to drench30days is needed to achieve a stable concentration of the drug, and after60days, the index still maintain good control, showing a good in the long-term efficacy. |
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