Study On The Pharmacognosy, Chemistry And Acute Toxicity Of Aconitum Soongaricum Stapf.

Objective: To study the pharmacognosy of Aconitum soongaricum Stapfsystematically; to confirm the components and to study the acute toxicity of Aconitumsoongaricum Stapf on mice, while investigating the acute toxicity, toxic signs, organcoefficient, median lethal dose (LD₅₀) and95%confidence interval on mice of A.soongaricum Stapf. Methods: Macroscopic and microscopic identifications were carriedout to observe the crude drug, fabric and powder, and high performance liquidchromatography （HPLC） and thin layer chromatograph （TLC） were used to analyze thealkaloids in A. soongaricum Stapf, and corresponding methods were set up to determinethe contents of the alkaloids. Different concentrations of A. soongaricum Stapf originalmedicinal powder suspension are given to mice, for observing and recording the activityand toxicity in mice, and at the same time, for computing the value of LD₅₀.Results: Thecharacteristics of A. soongaricum Stapf were spindle root,2-4roots, black brown skin andbluish violet sepals. The components of A. soongaricum Stapf were12-epi-napelline,songorine, neoline, songoramine and napelline, and also, songoramine and napelline werefound in it for the first time. The mice showed reduced activity, sweating, tremor andmouth breathing toxic symptoms after medicine administration about thirty min, and thepeak mortality rate was two hours. The median lethal dose was155mg/kg, and the95%confidence interval was137-175mg/kg. Conclusion: The results can be taken as thereference for identifying the species, analyzing the quality and laying down qualitystandard of the crude drug.