The Effects Of Aconitum Soongaricum Stapf. On Endogenous Metabolites In Rats

Objective: To study the changing regularity of Aconitum soongaricum Stapf. on themetabonomic profile of rats serum and urine with different time and dose and itsrelationship with blood biochemical indicators, providing indicators for clinical testing ofthe Aconitum soongaricum Stapf.. Methods: SD rats were administrated orally with0,21.25,42.50and85.00mg/kg Aconitum soongaricum Stapf for14days respectively. Afterdosing, serum and urine were collected and their1H nuclear magnetic resonance (NMR)spectra were acquired and all serums were taken blood biochemical analysis. SD rats wereadministrated orally with170mg/kg Aconitum soongaricum Stapf for once, the serum of0h,2h,6h,2d,3d,5d,7d,9d,11d,13d and14d were collected respectively and their1H nuclear magnetic resonance(NMR) spectra were acquired and all serum were takenblood biochemical analysis.Results: The serum levels of TC, TG, TP and ALB inadministered group were decreased with dose increased, there was significant difference inmiddle-dose and high-dose compared with control group(P<0.05), the levels of TC, TGwere decreased at6hours after dosing, while the levels of TP, ALB were decreased in2days. The serum levels of ALT, AST, LDH, TB and CREA in administered group wereincreased with dose increased, there was significant difference in middle-dose andhigh-dose compared with control group(P<0.05), the levels of CREA and LDH wereincreased at2hours after dosing, while the levels of TB were increased in2days. Theserum levels of GLC in low-dose and middle-dose group were decreased with doseincreased, while the levels of GLC in high-dose group were increased with dose increased,there was significant difference in middle-dose and high-dose compared with controlgroup (P<0.05). It was found that there have signification difference between the1H-NMRof serum and urine in different groups, the variability of metabolin in the serum ofdifferent dosing group were VLDL, LDL, glucose, lactic acid, pyruvic acid, alanine,glutamic acid, creatine and1-methyl-histidine. The variability of metabolin in the urine ofdifferent dosing group were isoleucine, α-oxoglutaric acid, citric acid, creatinine, taurine, hippuric acid, glycine, dimethylamine, trimethylamine and succinic acid. The variabilityof metabolin in the serum of different timing group were VLDL, LDL, glucose, lactic acid,creatine, trimethylamine oxide, glycine, glutamic acid, alanine, β-hydroxybutyric acid,pyruvic acid and acetoacetic acid. Conclusion: Aconitum soongaricum Stapf cansignificantly decrease the serum levels of TC, TG, TP and ALB and increase the serumlevels of ALT, AST, LDH, TB, GLC and CREA. The metabomic spectra of the rat serumand urine were closely associated with the time of toxic effects and the dose of theAconitum soongaricum Stapf. Different metabolic components were existed in the serumand urine of different groups, involving lipid metabolism, glucose metabolism, amino acidmetabolism and energy metabolism. The results of present studies suggest thatmetabonomic techniques can be used to characterize the metabolic consequence Aconitumsoongaricum Stapf, which can be used to elucidate the toxic of their effects andmechanism.