The Study On Medicinal Value And Reduce The Toxicity With Processed Of Aconitum Soongaricum Stapf In Xinjiang

Objective:To research the before and after processed in Aconitum soongaricum Stapf in Xinjiang alkaloid content and the change of the toxicity and pharmacological,determine the Aconitum soongaricum Stapf in Xinjiang processed in method.Methods:1.Used by reveed-phase HPLC before and after processed in Aconitum soongaricum Stapf in Xinjiang alkaloid content changes.2.By using mice following intraperitoneal injection method for determination LD50 of different processed products of Aconitum soongaricum Stapf.3.Used radiant heat stimulation and acetie acid indueed writhing test and investigated analgesic function of different processed products of Aconitum soongaricum Stapf.4.Observed the different processed products of Aconitum soongaricum Stapf to AA anti-inflammatory effect of rats.5.To observe the different processed products of Aconitum soongaricum Stapf to CIA anti-inflammatory effect of rats.Results:1.Aconitum soongaricum Stapf in Xinjiang was the aconitine content was higher,the content of Mesaconitine and Hypaconitine was low,processed products of double ester type of alkaloid content was reduced,single ester type alkaloid content increased.2.The toxicity order of different processed products of Aconitum soongaricum Stapf:Product>Literature method>Pharmacopoeia method(2010)>Kazakh method;The result showed that the LD50 were 3.079 2 g/kg,26.219 1 g/kg,10.587 3 g/kg and 39.870 2 g/kg respectively.3.(1)Compared with contrast group,The administration groups are able to significantly extend mice from radiation caused by thermal stimulation of mouse tail-flick latency except the low dose from Pharmacopoeia method(2010)and Kazakh method;(2)Compared with contrast group,The administration groups could reduced rat torsion times(P<0.01 or P<0.05),which had analgesic effects except the low dose from Kazakh method.4.(1)The swelling degree and arthritis index of Aconitum soongaricum Stapf and all of its processed products groups were lower(P<0.01 or P<0.05).(2)The content of Cort and IL-1? in serum of positive group,Aconitum soongaricum Stapf and all of its processed products groups were lower(P<0.01 or P<0.05).5.(1)The swelling degree of Aconitum soongaricum and all of its processed products groups were reduced.(2)By light microscopy observation,the treatment group synovial histopathological form had improved significantly.(3)Compared with model group,give medicine 6 weeks,the content of SA,IL-2 and TNF-? with The administration groups had significant differences(P<0.01 or P<0.05)except its Kazak method processed product group.Conclusion:(l)Aconitum soongaricum Stapf processing synergia from action mechanism and the diester-type alkaloids content reduced,single ester type alkaloid content increased related.(2)Acute toxicity and pharmacodynamics experiment shows that literature method processed products and pharmacopoeia method(2010)processed products of analgesia and anti-inflammatory effects are much better than Kazakh method processed products,But pharmacopoeia method(2010)processed products of toxic smaller than literature processed products.(3)Aconitum soongaricum Stapf after processing can reduce the toxicity and increase effect,has some of the medical values,The pharmacopoeia method(2010)processed products of acute toxicity,pharmacodynamics are better than the other two kinds of processing methods,So optimization pharmacopoeia method(2010)as Aconitum soongaricum Stapf reduce the toxicity and increase effect the processing technology.