The Studies Of Regulating Blood Lipid And Antioxidant Of Awei Capsules On Hyperlipemia Rats

Objective:To observe the effect of Awei capsules on regulating the blood lipid,prevention of fatty liver and improving the function of liver, improving the antioxidantcapacity on experimental hyperlipidemia rats. Methods: Thirty SD rats were randomlydivided into five groups: control group, model group and Awei capsules high-,medium-and low-dose group. Except the control group, other groups were fed with fat-rich forage.Hgih-, medium-and Low-dose groups were given aqueous solution of the sodiumcarboxymethyl cellulose of Awei capsules contents. It were1.28,0.64,0.32g/kgrespectively. The rats of control group and model group were taken aqueous solution ofthe sodium carboxymethyl cellulose. It were seven weeks continuously, six days a week.Record the water intake, food intake of rats daily, and weigh weight of rats weekly. Ratswere not given any food, but water for24hours before taken the blood. Rats were injected10%chloral hydrate to anesthesia. Taken blood from the abdominal aorta of rats till toeuthanasia. Separate serum. Taken the part of liver tissue into10%neutral Formalinsolution for microscopic detection, and took part of liver saved into the liquid nitrogen forcompleting the study of HMG-CoA RNA expression levels by electrophoresis andfluorescence quantitative PCR. The indicators of detecting: water intake, food intake, ratsweight, liver weight, brain weight, heart weight, organ coefficient; liver appearance andcharacter of the pathologic morphology of liver; TC, TG, HDL-C, LDL-C, AST, ALT,GLU, NO, MDA, activity of SOD in the serum; and the expression level of HMG-CoA inrat’s liver. Results: Compared with the blank group, the model group of male and femalerats liver color was yellower; it could be observed lipid droplets and inflammatory cellinfiltration phenomenon on liver cells by optical microscope detection; the difference ofliver coefficient, TC, LDL-C, ALT, MDA, NO, SOD activity and AST female TG wasstatistically significant(P<0.05,P<0.01). Liver coefficient,TC, TG, LDL-C, AST, ALT,MDA and NO of model group were higher, SOD activity was lower. Compared with the model group, the intracellular lipid droplets and inflammatory cell infiltrationphenomenon of liver were lower in Awei capsules three dose groups by the opticalmicroscopy; liver coefficient of three dose groups on female rats, TC of female rats inthree dose groups, TG of male and female rats in high dose group, LDL-C of male andfemale rats in three dose groups, AST of female rats in three dose groups, ALT of femalerats in high dose group, ALT of male rats in high dose group, MDA in three dose groups,NO in high dose group, SOD in high-and middle-dose groups, the differences of themhad significant difference (P <0.05, P <0.01). The differences of other index, had nostatistical significance. The other indexs of Awei capsules groups have the trend to chosingto the control group. Conclusion: Blood lipids and liver morphology, microscopicstructure, function in hyperlipemia rats were abnormal in model group, so thehyperlipidemia model of rats was successful. Awei capsules can regulate blood lipid,increase the antioxidant capacity, prevente of fatty liver and improve liver function. But inthis study, it was not showed that the intensity of effect had correlation trend with the doseof Awei capsules.