Study On The Relationship Of Infertility With Ureaplasma Urealyticum And Its Biovars

Objective:(1) To compare the differences of Ureaplasma urealyticum (UU) and subtype infection rate between female infertility group and control group.(2) To compare the macrophage migration inhibitory factor (MIF) expression level of THP-1cells infected by different concentration and biogroup of UU, and to explore the pathogenicity of different concentration and biogroup of UU.Methods:(1)120cases of female infertility patients who went to the second hospital of Tianjin Medical Univercity in2011were enrolled in the study. Another120cases of normal female were selected as control group. Their specimens of cervix secretion were inoculated and cultured in the liquid culture; and the positive specimens were further confirmed by solid culture. Final UU infection rates were compared between the infertility group and control group.(2) DNA from specimen with UU positive by solid culture was extracted. A PCR method was used to separate two different UU subtype; and the infection rates of different subtypes were compared between the infertility group and control group.(3) Different UU subtype cultures were used to infect the THP-1cells. The final UU concentrations which infected THP-1cells were104,105and106CCU/ml. At6h,12h,18h, and24h after infection, the MIF expression level in the supernatants was detected by ELISA. THP-1cell infected by culture medium was served as the control group. The MIF expression level was compared between the UU infection group and control group, the UU1infection group and UU2infection group, respectively.Results:(1) The infection rate was60.8%(73/120) and35.0%(42/120) in the infertility group and control group, respectively by liquid culture. The final infection rate was55.0%(66/120) and30.0%(36/120) in the infertility group and control group, respectively by solid culture. The difference has statistical significance (χ2=15.35, P<0.05).(2) Two UU subtypes were detected by PCR. Biogroup1was positive in25samples (20.8%), biogroup2was positive in55samples (45.8%), and both biogroup1and2 positive was14(11.7%) in the infertility group; biogroup1was positive in21samples (17.5%), biogroup2was positive in26samples (21.7%), and both biogroup1and2positive was10(8.3%) in the control group. There was significant difference between the infertility group and control group about positive rate of biogroup2(χ2=15.67,P<0.01).(3) After infection with UU1culture at concentration of104CCU/ml, there was no significant difference of MIF expression between the infection group and the control group (P>0.05); At24h after infection with UU1culture at concentration of105CCU/ml and106CCU/ml, the MIF expression increased significantly than the control group (P<0.05).(4) After infection with UU2culture at concentration of104CCU/ml, there was no significant difference of MIF expression between the infection group and the control group (P>0.05); At18h and24h after infection with UU2culture at concentration of105CCU/ml and106CCU/ml, there was significant difference of MIF expression between the UU2infection group and control group (P<0.05)(5) At18h and24h after infection with UU biogroup culture at concentration of105CCU/ml and106CCU/ml, there was significant difference of MIF expression between the UU1infection group and UU2infection group (P<0.05)Conclusions:(1) The UU infection rate is higher in the infertility group than the control group. It is proved that UU infection is closely related to female infertility.(2) The UU biogroup2infection rate is higher in the inferlity group than the control group, and there is no statistical difference about biogroup1infection rate between two groups. It is proved that UU2biogroup is pathogenic bacteria and UU1biogroup is normal carried bacteria.(3) UU pathogenicity is associated with UU subtypes and infection concentration. More than104CCU/ml concentrations have the pathogenic feature; the UU2biogroup has stronger pathogenicity than the UU1biogroup,...