| Objectives:1.To investigate the change and clnical significance of KL-6level in serum of patients with acute exacerbation of bronchial asthma.2.To explore the relationship of asthmatic patients with acute exacerbation KL-6、T-IgE、FEV1%pred and FeNO.Methods:1.56patients with acute exacerbation of bronchial asthma were divided into3groups in accordance with the diagnostic criteria for classification of acute exacerbation of bronchial of asthma,32normal subjects as control group.56patients with acute exacerbation of bronchial asthma were from Tianjin first central hospital from2011.01to2011.11. Every patient was informed about study protocol and procedures and signed informed consent form.2.ELISA detection of KL-6in serum:56patients of asthmatic patients were taken blood before treatment, the experiment was carried out in accordance to the instructions supplied with the kits.3.Detection of FEV1%pred、FeNO of asthmatic patients1)40cases of asthma patients measured FEV1%pred,Pulmonary function was measured by lung function cryoscope, and pulmonary function and serum concentrations of KL-6correlation analysis.2)40cases of asthmatic patients were measured FeNO, and were compared with the level of KL-6.4.Detection of EOS、T-IgE in serum of asthma-tic patients:56cases of asthma in hospital laboratory determination of the line EOS,56patients with asthma were treated with T-IgE determination, and were compared with the level of KL-6respectively.Results:Acute exacerbation of bronchial asthma in the concentration of serum KL-6:KL-6for the control group293.15±89.54U/ml; KL-6for the case group:432.18±85.13U/ml;mild group KL-6:351.13±72.86U/ml;moderate group KL-6:55.78±93.23U/ml; severe group KL-6:625.14±11.32U/ml. The comparison between groups was statistically significant (F=20.834, P=0.000), after the ONE-ANOVA analysis derived:mild and moderate groups was statistically significant (P=0.031); moderate group and severe group were significantly different (P0.020);severe group and mild group were significantly different (P=0.000). The control group compared with the case group were significantly different (P=0.000); the control group and the mild group were significantly different (P=0.047); the control group and the moderate group were significantly different (P=0.008); the control group and severe group were significantly different (P=0.000). For statistical analysis of T-IgE between the control group and the case group,P=0.134, were not statistically significant, and there were not statistically significant among the case groups.Statistical analysis of the serum of the EOS, P=0.145, were not statistically significant,and there were not statistically significant among the case groups. FeNO values after the ONE-ANOVA analysis between the control and case groups=0.000, statistically significant, by ONE-ANOVA analysis between the case groups:mild group VS moderate group P=0.013, was statistically significant; mild group VS severe group P=0.000, with statistical significance; moderate group VS severe group P=0.032, was statistically significant.56patients with acute exacerbation of asthma FEV1%pred of patients with ONE-ANOVA analysis was P=0.000, was statistically significant, the ONE-ANOVA analysis derived:mild group VS moderate group P=0.027, was statistically significant; moderate group VS severe group P=0.013, was statistically significant; mild group VS severe group P=0.000, was statistically significant.Acute exacerbation of bronchial asthma with serum KL-6、 EOS no correlation, r=0.199, P=0.14>0.05; KL-6and T-IgE had no correlation,1=0.127,P=0.351>0.05; KL-6was negatively correlatd with the FEV1%pred, r=0.615,P=0.007; KL-6was positively correlated with FeNO, r=0.618,P=0.000<0.05.Conclusions:1. The concentration of KL-6increases in the acute exacerbation of asthmatic patients, it may be involved in the pathogenesis of asthma.2. The concentration of KL-6in serum of acute asthma patients is closely related with the severity of bronchial asthma, it maybe as an asthma assistant diagnosis and severity assessment indicators. |
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