| Objective:Observed the relationship among Left Ventricular Hypertrophy (LVH) and Blood Stasis Syndrome and analysis of monocyte chemoattractant protein-1(MCP-1) gene polymorphism in Hemodialysis Patients,to explore the risk factors associated with LVH in hemodialysis patients, analysis of genetic susceptibility factors for LVH and blood stasis.Methods:142patient cases of Hemodialysis were included. Dividing the cases into LVH group and non-LVH group according to the LVMI. valuing the blood stasis of142cases, Measuring the MCP-1gene regulatory region-2518A/G gene polymorphisms differences in hemodialysis patients by using the polymerase chain reaction restriction fragment length polymorphism (PC-RRFLP), Analysis of the relationship between the degree of LVH and blood stasis score, and MCP-1gene polymorphism.Results:1.142hemodialysis patients with LVH in97cases and incidence rate of68.3%(97/142)2.LVH group on systolic blood pressure, diastolic blood pressure,and Ultrafiltration Volume was significantly higher than non-LVH group, and Hb content was significantly lower than non-LVH group, and no statistical differences between the two groups in age, gender, duration of dialysis and predialysis creatinine, albumin, calcium, phosphorus, total cholesterol, triglyceride and parathyroid hormone levels.3.LVH group of97cases, the average score of blood stasis was33.90±10.84(cover from13to62), non-LVH group of45cases, the average score of blood stasis was17.24±5.70(cover from8to29), The former being significantly higher than the latter (P<0.05).4.There was a significant correlation between the Blood stasis score, and ultrafiltration volume, HB,the TC, TG (r=0.236,-0.231,0.212,0.176, P<0.05) 5. Three genotypes (AA, AG and GG) were detected in the MCP-1-2518gene polymorphism,AA, AG and the GG three kinds of genotypes frequency respectively10.6%(15/142),54.9%(78/142),34.5%(49/142) in hemodialysis patients, in the normal control group were12.2%(10/82),56.1%(46/82),31.7%(26/82); no significant difference of genotypes in two groups (P>0.05). A and G allele frequency were38.0%(108/284),62.0%(176/284) in the hemodialysis group, in the normal control group were40.2%(66/164),59.8%(98/164); there was no significant difference between the allele frequencies in two groups (P>0.05).6.The distribution of MCP-1-2518gene polymorphism in hemodialysis patients with different underlying diseases, AA, AG and the GG genotypes were10.3%(7/68),57.3%(39/68),32.4%(22/68) in patients with chronic glomerulonephritis group, in the diabetic nephropathy group were10.6%(5/47),51.1%(24/47),38.3%(18/47), in hypertensive renal injury group were11.1%(3/27),55.6%(15/27),33.3%(9/27); The distribution frequency of A and G alleles were39.0%(53/136),61.0%(83/136) in the chronic glomerulonephritis group, were36.2%(34/94),63.8%(60/94) in the diabetic nephropathy group, in hypertensive renal injury group were38.9%(21/54),61.1%(33/54); there was no significant difference in allele frequencies among the three groups.7.The incidence of AA, AG, the GG genotypes were8.3%(8/97),51.5%(50/97),40.2%(39/57) in LVH group, were15.6%(7/45),62.2%(28/45),22.2%(10/45) in non-LVH group, no significant differences between the two groups; The distribution frequency of A and G allele were34.0%(66/194),66.0%(128/194) in LVH group, were46.7%(42/90),53.3%(48/90) in the non-LVH group, there are significant differences between the two groups (P<0.05).8. The relationship between MCP-1gene polymorphism and blood stasis syndrome:GG genotypes in blood stasis score29.26±8.27; and AA genotype blood stasis score was23.13±7.18, both have a statistics difference (P<0.05).9. The relationship between MCP-1gene polymorphism and LVH was a statistically significant difference (P<0.05), LVH group of MCP-1gene-2518G allele frequency was significantly higher than non-LVH group.10. Logistic regression results show that the risk factors of LVH was MCP-1gene polymorphism, ultrafiltration volume, blood stasis level and systolic blood pressure.Conclusion:1. The LVH incidence rate was68.3%in Hemodialysis patients, There was a negatively correlated between LVMI and Hb content, and a positively correlated between LVMI and ultrafiltration volume, systolic’blood pressure in Hemodialysis patients.2. There was positively correlated between the degree of LVH and Blood Stasis Syndrome in Hemodialysis Patients.3. There was negatively correlated between blood stasis score and Hb, and a positively correlated blood stasis score with ultrafiltration volume,TC, TG in Hemodialysis patients.4. The MCP-1-2518G allele were more likely to have left ventricular hypertrophy,5.The MCP-1-2518GG genotype more susceptible to blood stasis. |
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