Study On The Chemical Composition And Biological Activities Of Foeniculum Vulgare Mill

Foeniculum vulgare Mill., commonly known as fennel, is dried fruit of the plant of the genus Foeniculum vulgare, Apiaceae. The whole plant can be use as medicinal. It is one of general-adopted and important Chinese medicine. According to the Chinese pharmacopoeia (2010Edition), it could dispel cold, relieving pain. And literature reports it have anti-inflammation, antioxidant, analgesic and anti-hepatic fibrosis effects. In order to explore the substances and mechanism of anti-hepatic fibrosis, acetic acid-induced writhing in mice model, acetaldehyde-induced hepatic stellate cell apoptosis experimental xylene-induced ear edema model and mouse plasma antioxidant biochemical indicators were study. Study from the chemical plants, animals and cellular level pharmacological, the chemical composition of fennel and biological activities were initially clear. This research was mainly focused on phytochemistry, animal and cell pharmacology. The research can divide into four sections as follow.1. Chemistry constituents of fennel42chemical constituents had been isolated by Silica Gel column, Sephadex LH-20, Preparative TLC, Preparative HPLC chromatography and the spectroscopic methods. And22compounds had been identified by detailed spectroscopic analyses on the baise of1H-NMR,13C-NMR and2D-NMR. Including9compounds isolated from this genus for the first time.2. Acetaldehyde-induced hepatic stellate cell apoptosis experimentalIn the experimental of acetaldehyde-induced hepatic stellate cell apoptosis, fennel ethanol total extract, volatile oil, trans-anethole, and some compounds were screening. Results showed that the total extract, volatile oil, trans-anethole to some extent inhibition acetaldehyde-induced HSC-T6cell proliferation. Volatile oil was the most active compound.3. Anti-inflammatory, analgesic activityXylene-induced ear edema mice experiments show fennel has a strong anti-inflammatory activity. Acetic acid writhing experiment prove that the volatile oil and trans-anethole have a strong analgesic activity, which consistent with and provide the basis for traditional medicinal. Volatile oil was the most active compounds.4. Antioxidant activityBy examining the effects of fennel on mouse plasma antioxidant enzymes SOD、MDA、 CAT、HDL-C and TG activity shows that volatile oil of fennel and trans-anethole have a strong antioxidant activity.