Preliminary Study On The Mechanisms Of Hepatitis B Virus Paternal-fetal Vertical Transmission

Objective: To study on the mechanism of HBV infection through detecting HBV(hepatitis B virus) membrane receptor in sperm, and then, observed the changes ofsemen parameters caused by hepatitis B virus infection. What’s more, establish thetheoretical supports of HBV block in vitro.Methods: Normal semen were cultured with HBV positive serum after differenttreatment. Experimental group are divided into the following four groups: Group A:normal semen; Group B: HBV serum and normal semen; Group C: HBV positiveserum and normal semen; Group D: ASGPR Mab（asialoglycoprotein receptormonoclonal antibody），HBV positive serum and normal semen. Sperm motility (a+b)were observed and recorded each group every hour. The flow cytometry analysiswere performed for sample of group C and D to detect the HBsAg and HBcAg insperm membrane surface after24-hours.Results: Sprem motility after1hour’s cocultured, group A>D，C>B，C>D(P<0.05).2hours A>C,B>C,D>C(P<0.05).3hours A>D>B>C(P<0.05).4-6hours A> B=D>C (P<0.05).7hours A>B,A>C,B>C,D>C(P<0.05). The HBcAg was no differencebetween group C and group D, but the HBsAg was higher in group C than group D.Conclusion: HBV infection can lead to decrease of sperm motility (a+b)，ASGPRMab can effectively prevent the sperm parameters changing caused by HBV. Foranother, ASGPR is one of the most important membrane protein receptor to HBVpaternal-fetal vertical transmission. Furthermore inhibit ASGPR in vitro maybe agood method to block the HBV paternal-fetal vertical transmission.