| Objective:Through the establishment of rat model of ulcerative colitis to study the influence of the qinghuachangyin on the expression of general condition,the colon changes observed by naked eyes,pathologic observation of colon tissues,serum inflammatory cytokines (tumor necrosis factor-a, interleukin-6, interleukin-8, interleukin-10),and compared with mesalazine treatment effect,explore the treatment of UC mechanism,provide new scientifically theoretical basis for clinical application,new methods and ideas for UC treatment.Methods:60SPF healthy Wistar rats,half male and half female,were randomly divided into normal group, model control group,high-dose qinghuachangyin group,in-dose qinghuachangyin group, low-dose qinghuachangyin group and mesalazine group,Rat models were established by immune complex method in all groups except normal group.Three days after model construction, rats were treated with high, medium, low doses of qinghuachangyin and mesalazine by gavage, whereas rats in normal and model control groups were given distilled water by gavage, each group was treated daily for28days.Results:(A).General observation:Before treatment, normal group was better than the other model groups,all groups presented diarrhea swiftly which lasted to the end of experiment,part of them appeared blood stool, mucous stool and pus stool. After treatment, high-dose qinghuachangyin group and mesalazine group were improved in general condition, in-dose qinghuachangyin group and low-dose qinghuachangyin group were similar to model control group,the effect was not obvious.1. Weight:Before treatment,weight of the model groups were significantly lower than normal group (P<0.05), rat models among groups without significant difference ((P>0.05).After treatment, high-dose qinghuachangyin group and mesalazine group were significantly higher than model control group、in-dose qinghuachangyin group and low-dose qinghuachangyin group (P <0.05).2. DAI:Before treatment,DAI of the model groups were significantly higher than the normal group (P<0.01), no significant difference among every model group ((P>0.05).After treatment, high-dose qinghuachangyin group and mesalazine group were significantly lower than model control group,in-dose qinghuachangyin group and low-dose qinghuachangyin group (P <0.01).(B).The colon changes observed by naked eyes:Before treatment,rat model of colon mucosa hyperemia points, accompanied by erosion, ulcers, mural thickening of intestine, etc. After treatment, the colon tissue lesions of high-dose qinghuachangyin group and mesalazine group were improved obviously, there were little erosion, ulceration has disappeared, or the healing scar of ulcer could be seen, mural thickening of intestine was not seen; in-dose qinghuachangyin group and low-dose qinghuachangyin group were similar to model control group.(C).Pathologic observation of colon tissues:Before treatment,rat model of colon expressed erosion, ulceration and depth of myometrial, edge gland necrosis, granulation tissue,A large number of lymphocytes, plasma cells, macrophages were scattered in lamina propria and submucosa.After treatment, the colon tissue lesions of high-dose qinghuachangyin group and mesalazine group were improved, only little erosions existed, ulcers has disappeared; in-dose qinghuachangyin group and low-dose qinghuachangyin group were no significant changes.(D).Changes of inflammatory cytokines in serum1.TNF-a:Model control group was significantly higher than normal group (P<0.01); high-dose qinghuachangyin group and mesalazine group were significantly lower than model control group (P<0.01),-in-dose qinghuachangyin group and low-dose qinghuachangyin group compared with model control group had no significant difference(P>0.05); mesalazine group was significantly lower than in-dose qinghuachangyin group and low-dose qinghuachangyin group (P <0.01), but compared with high-dose qinghuachangyin group had no significant difference (P>0.05); high-dose qinghuachangyin group was significantly lower than in-dose qinghuachangyin group and low-dose qinghuachangyin group (P<0.01).2.IL-6:Model control group was significantly higher than normal group (P<0.01); high-dose qinghuachangyin group and mesalazine group were significantly lower than model control group (P<0.01); in-dose qinghuachangyin group and low-dose qinghuachangyin group compared with mode! control group had no significant difference(P>0.05); mesalazine group was significantly lower than in-dose qinghuachangyin group and low-dose qinghuachangyin group (P <0.01), but compared with high-dose qinghuachangyin group had no significant difference (P> 0.05); high-dose qinghuachangyin group was significantly lower than in-dose qinghuachangyin group and low-dose qinghuachangyin group (P<0.01).3.IL-8:Model control group was significantly higher than normal group (P<0.01); high-dose qinghuachangyin group and mesalazine group were significantly lower than model control group (P<0.01); in-dose qinghuachangyin group and low-dose qinghuachangyin group compared with model control group had no significant difference(P>0.05); mesalazine group was significantly lower than in-dose qinghuachangyin group and low-dose qinghuachangyin group (P <0.01), but compared with high-dose qinghuachangyin group had no significant difference (P>0.05); high-dose qinghuachangyin group was significantly lower than in-dose qinghuachangyin group and low-dose qinghuachangyin group (P<0.01).4.IL-10:Model control group was significantly lower than normal group (P<0.01); high-dose qinghuachangyin group and mesalazine group were significantly higher than model control group (P<0.01); in-dose qinghuachangyin group and low-dose qinghuachangyin group compared with model control group had no significant difference(P>0.05); mesalazine group was significantly higher than in-dose qinghuachangyin group and low-dose qinghuachangyin group (P <0.01), compared with high-dose qinghuachangyin group had no significant difference (P>0.05);high-dose qinghuachangyin group was significantly higher than in-dose’qinghuachangyin group and low-dose qinghuachangyin group (P<0.01).Conclusion:(A).The experiment succeed in preparing the UC rat model, rat models in general condition, observed by naked eyes, histopathology, inflammatory cytokines (TNF-a, IL-6, IL-8, IL-10) had abnormal expression.(B).The rats’general situation, the colon changes observed by naked eyes and the histopathology all shows that high-dose qinghuachangyin can repair diseased colonic mucosa tissue obviously.(C). After treatment of UC by high-dose qinghuachangyin, the proinflammatory cytokine levels(TNF-a, IL-6,IL-8)was decreased, the Anti-inflammatory cytokines levels(IL-10)was raised. (D).The experimental study showed that high-dose qinghuachangyin is an effective drug for UC treatment, and regulating the balance of inflammatory cytokines may be one of the UC mechanisms. |
PDF Full Text Request