The Correlation Research About Ras/Raf/MAPK Signaling Pathways With The Pathological Scar And Scar Cancer

Objective:Explore the correlation about the Ras/Raf/MAPK signaling pathway and the pathway downstream, analysis of pathological scar cancer-related factors, explore the feasibility of scar cancer targeted the rapy targets, to provide a theoretical basis for clinical targeted therapy to curb the pathological scar cancer and scar carcinoma.Methods:(1) Use the scanning confocal microscopy, immunofluorescence double labeling of the15cases of pathological scars and25cases of scar cancer paraffin tissue sections of the K-ras, H-ras, N-ras and CyclinD1, collecting three-dimensionalimang.(2) the Protein expression of MAPK, CyclinD1were detected10cases of normal skin tissue,15cases of pathological scarsd and20cases of scar cancer three groups of organizations by the immunohistochemical method of S-P.(3) The expression of MAPKmRNA, CyclinD1mRNA were detected by in situ hybridization.(4) The analysis of image were combinted, and calculated the optical density and positive area indicators seized in the inspection organization, and all data was input into the computer and statistically analyzed with SPSS (16.0) software.Results:(1) The results of the laser scanning confocal microscopy:the expression of K-Ras, H-Ras, N-Ras and CyclinD1in pathological scar epithelium showed weak fluorescence, for the weak expression; It showed strong fluorescence expression in scar carcinoma,for the stron gexpression. The expression of sites and intensity are consistent with the results of the immunohistochemical markers.(2) MAPK and its mRNA showed negative expression in normal epidermis, showed weakly positive expression in pathological scar epithelium, strongly positive expression in scar carcinoma. The expression of levels (positive area)and intensity(mean optical densit) differences were statistically significant (P<0.01) by compared with normal skin group and skin scar group. But the differences of expression was no statistically significant between normal skin group and pathological skin scar group.(3)CyclinD1and its mRNA showed negative expression in normal skin epidermis, weakly positive expression in pathological scar epithelium, strongly positive expression in scar carcinoma group. The expression of levels (positive area)and intensity(mean optical densit) differences were statistically significant (P<0.01), by compared with normal skin group and skin scar group. But the differences of expression was no statistically significant between normal skin group and pathological skin scar group.(4) Correlated analysis showed:It was positive correlation the expression levels of MAPK and its mRNA, CyclinD1and its mRNA, CyclinDl and MAPK protein, CyclinD1mRNA and MAPK mRNA.Conclusion:(1) In the pathway, the Ras protein, MAPK protein and its mRNA, CyclinD1protein and its mRNA showed weakly positive expression in the epithliun of pathological scar, suggested that it is limited about the significance of the pathological scar carcinogenesis.(2) In the pathway, the Ras protein,MAPK protein and its mRNA, CyclinDl protein and its mRNA showed strong positive expression in the scar cancer tissue, which may be play important roles in the occurrence of scar carcinoma. and a variety of genes play a synergistic.(3)In the pathway, the Ras protein, MAPK protein and its mRNA, CyclinD1protein and its mRNA showed strong positive expression in the scar cancer tissue,Fitted as a target point of tragefed therapy, suggested that which was subsistent to provide theoretical basisand feasibility about the scar cancer targeted therapy.