| Fat transplant has been carried out about one hundred years of history The position in the field of plastic surgery is particularly important。Because of its rich source, Drawing easy,simple, good filling shape, no rejection, etc., it is used for filling the facial sunken deformity, Long temporal, nose job and breast augmentation and other cosmetic plastic surgery. However, the survival rate of transplanted fat is lower, usually30%to60%。The main reason is the fat tissue transplantation failed to effectively establish the blood supply in the affected area, Lead to transplantation of adipose tissue ischemia, necrosis and absorption.Therefore, how to accelerate the establishment of the blood supply of grafts has become a research focus in fat tissue transplantation.objective:In this study, by establishing rat model of fat transplantation, VEGF and bFGF in the complex is added rat fat particles autologous。The use of the role of VEGF and bFGF in promoting blood vessel growth and accelerating reconstruction of local blood circulation。To study the early angiogenesis of Transplanted in vivo and its impact on survival of free fat transplantation.Methods:The wistar50rats were randomly divided into five groups, Saline control group10, the second experimental group of VEGF10, the third experimental group, bFGF group10, the fourth experimental group VEGF, bFGF combined treatment group10,(Compared with the control group and VEGF, bFGF, combined treatment group) plus another control group that small doses of VEGF +bFGF Treatment10.Removed the abdominal omental fat total about2.0g, Cut the fat into pieces with fine granular micro scissors, about1-2mm, washed with20ml syringe, the other group free fat preparation above.In the back to make a small longitudinal skin incision, the fat particles have been injected into the back compartment, the incision sutured back.Four groups in each group were randomly selected in all five wistar rats, drawn in after15,30days.The fat transplantation be seen dark yellow.It is enveloped by layer of fibrous tissue boundaries clear.To remove the fat grafts transplanted into the precision measurement of balance scale for body weight, read and record data.After paraffin-embedded10%formalin-fixed, sliced, alternate CD34immunohistochemical staining.Results:1. The the comparison of residual weight of VEGF+of bFGF (0.1μg) fat particles transplantation group and control group:15days of graft residual correction of the weight of statistical analysis, t-testcomparison between groups.The experimental group and control group, P <0.05, between the two groups were significantly different. Experimental group and VEGF group F=2.25, P=0.03, P<0.05between the two groups were significantly different. Experimental group andbFGF group F=1.68, P=0.03, P <0.05between the two groups were significantly different. bFGF and VEGF group F=1.23, P=0.86, P>0.05between the two groups, no significant difference.30days of graft residual correction of the weight of statistical analysis, the experimental group and control group, P<0.05, significant difference between the two groups. Experimental group and VEGF group F=1.12, P=0.03, P<0.05between the two groups were significantly different. Experimental group and bFGF group F=2.85, P=0.04, P<0.05between the two groups were significantly different. bFGF and VEGF group F=2.54, P=0.56, P>0.05between the two groups, no significant difference.2. Comparison of VEGF+of bFGF (0.1μg) fat particles transplantation group andcontrol group (×400) of CD34staining:Between the staining of vascular endothelial cell number in the fat grafts after30daysof CD34staining of VEGF+bFGF (0.1μg) handle fat particles transplant group(Figure12) and control group (Figure9) significant difference of VEGF+bFGF (each0.1μg) handle fat particles transplant group (Figure12) positive staining ofmore VEGF+bFGF (0.1μg) handle fat particles transplant group (Figure21)compared with VEGF+bFGF (each0.05μg) of the fat particles transplant group(Figure22) is slightly higher number of positively stained cells.3. Comparison of VEGF+of bFGF (0.1μg) fat particles transplantation group andcontrol group (×400) staining of CD34mean microvessel density:30days of graft microvessel count analysis, the experimental group and blank control group, F=1.16, P<0.05between the two groups were significantly different.Experimental group and VEGF group F=2.18, P<0.05between the two groupswere significantly different. Experimental group and bFGF group F=2.47, P<0.05between the two groups were significantly different. bFGF and VEGF group F=1.13,P=0.81, P>0.05between the two groups, no significant difference.4. Residual weight after transplantation of fat particles of the different contrast of VEGF in doses of basic fibroblast growth factor:15days of graft residual correction of the weight of statistical analysis, t-test forcomparison between groups. Two groups, with the blank control group, P<0.05between the two groups were significantly different. Low-dose group and blank group, P<0.05between the two groups were significantly different. High-dose group and blank group, P<0.05between the two groups have significant difference between low-dose group and high-dose group, P<0.05, significant differencebetween the two groups.30days of graft residual correction of the weight of statistical analysis, two groups with the control group, P<0.05, significant difference between the two groups. Low-dose group and blank group, P<0.05between the two groups were significantly different. High-dose group and blank group, P<0.05between the two groups havesignificant difference between low-dose group and high-dose group, P<0.05,significant difference between the two groups.5. Contrast of different doses of VEGF+bFGF fat particles transplant group (×400) staining of CD34mean microvessel density:30days of graft microvessel count analysis, the low dose group and high-dose group and the control group, P<0.05, significant difference between the two groups. High-dose group and low-dose group, P<0.05between the two groups were significantly different.Conclusion:(1)establish an effective model of neovascularization,Freshly isolated interstitial blood vessels can be effective in inducing angiogenesis.Greater omentum as a source of fat transplantation because of the greater omentum dense capillary architecture can improve the blood supply to the ischemic organ, blood vessel connections between the two structures quickly.Germany view that Liebermann Meffert, greater omentum and spleen with the same region originated in the gastric wall, they all have the function of phagocytosis formation of new blood capillaries.On the clinical application of the forward-looking, with the development of minimally invasive techniques, body fat filling in clinical applications provides very good idea.(2) the fat graft vascular biology show that Some cytokines play an important role in improving the survival of ischemic tissue. VEGF is a strong direct vascular inducible factor, early the most important contributing factor for angiogenesis, a highly conservative, and to stimulate the migration of endothelial cells in vitro, induced angiogenesis in vivo, maintaining the normal state of blood vessels, increase vascular endothelial permeability, play an important role in angiogenesis.BFGF can compensate for this deficiency of VEGF.It can promote constitute the main component of the blood vessels cells such as fibroblasts, smooth muscle cells and vascular endothelial cells and other value-added, and to achieve the effect of coordinated growth.In this study, the experimental model of fat transplantation has broad prospects. The combination of VEGF and bFGF to study adipose tissue revascularization, the purpose is to explor a new new method for improving transplantation of adipose tissue survival. VEGF and bFGF composite transplanted fat promote blood vessel growth and improve the survival rate of transplanted fat.(3) Joint application of VEGF and bFGF to study adipose tissue revascularization in the grasp dose provides a reference for clinical applications. VEGF+bFGF (0.1μg) fat particles transplant group has more significant role than in the low dose group in promoting fat growth. |
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