| 20-Hydroxyeicosatetraenoic acid (20-HETE), a ω-hydroxylation product ofarachidonic acid catalyzed by cytochrome P4504A (CYP4A). The production of20-HETE is increased during ischemia-reperfusion and inhibition of20-HETEproduction has been shown to reduce infarct size caused by ischemia. Our previousstudies show that20-HETE stimulates NADPH oxidase-derived superoxideproduction via a PKC-dependent mechanism in cardiomyocytes. This study aimedat discovering the molecular mechanism underlining the action of20-HETE duringischemia-reperfusion, and the main findings are showed below:⑴The intraceelllarROS content is detected by Dihydroethidium-Superoxide anion fluorescent detectingprobe.⑵By spectrophotometric method for the determination of cardiac tissuehomogenate supernatant of MDA,20-HETE significantly increased ROS and Lipidperoxidation by21%and20%respectively.⑶The RT-PCR results show that20-HETE compared to IR, myocardial cells in NADPH oxidase subunit Nox2mRNAexpression were increased by76%. In summary,20-HETE could contribute to thecardiac injury during myocardial ischemia. |
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