Neuroprotective Effects Of Adhatoda Vasica Nees Of Tibet Medicine In The Rabit Model Of Cerebral Ischemia-Reperfusion Injury

Objective:To observe neuroprotective effects of Adhatoda vasica Nees of Tibet medicine on cerebral ischemia reperfusion injury in rats and the influence of expression of the malondialdehyde and matrix metalloproteinase-9(mmp-9).Method:one hundred and ninety-eight healthy male Wistar rats to establish cerebral ischemia reperfusion model with middle cerebral artery thread embolism method, which were randomly divided into the sham-operation group, model group and the treatment group(Adhatoda vasica Nees treatment group). According to reperfusion time interval of6h,24h,48h,72h and120h after cerebral ischemia reperfusion injury, The model group and the treatment group were divided into five subgroups. There were eighteen rats in each subgroups and the sham operation-group. The treatment groups were given Adhatoda vasica Nees after the cerebral ischemia in1h, at dose of1.5mL/100g/time and after24h were given1.5mL/100g/d. The other groups were given the same dose of water. And then observed neurological deficit scores, the infarct sizes, the apoptotic cells and the malondialdehyde and matrix metalloproteinase-9of every group at the different time points.Results:The Wistar rats of sham group were healthy without any neurological deficit symptoms, and the nerve cells of the model group and treatment group have the infarct formation, and obvious edema in the zone of right cerebral. Cytoplasmic staining become shallow and nucleus stained darkly and become concentrated. The malondialdehyde and matrix metalloproteinase-9in treatment group and model group had been increased to varying degrees in the ischemia reperfusion. Compared with model group, the expression of malondialdehyde and matrix metalloproteinase-9, neurological deficit scores, infarct sizes, apoptotic cells of the treatment group decreased significantly, the difference was statistically significant (P<0.05).Conclusion:It is hypothesized that Adhatoda vasica Nees could improve the symptoms of neurological deficit after cerebral ischemia reperfusion injury, lower degeneration and necrosis of brain tissue and other pathological damage, reduce the edema of the nerve cells, shrink the volume of cerebral infarction, inhibit the neuronal apoptosis by down-regulation the expression of malondialdehyde and matrix metalloproteinase-9. The results suggest that Adhatoda vasica Nees plays a neuroprotective role in cerebral ischemia injury in rats.