To Explore The Mechanism Of The Apoptosis Of The Human Gastric Cancer Cell Line BGC-823via Notch Signaling In Vitro

Objective To investigate the apoptosis mechanism of the human gastric cancer cell line BGC-823via Notch signaling pathway.Methods DAPT, a γ-secretase inhibitor, was used to treat the human gastric cancer cell line BGC-823with different concentration or time, the effects of DAPT on the growth of the BGC-823were analyzed by MTT assay. The apoptotic rate was observed by flow cytometry (FCM). Real-time quantitative PCR and Western Blot were used to detect the mRNA and the protein expression changes of Notchl, Caspase8and Caspase9.Results The early apoptotic rate of BGC-823increased and performed a dose-time dependent manner after treatment of DAPT. except the group treated with l μmol/L DAPT for48h did not reach statistical significance compared with the control group (P>0.05).The mRNA and the protein expressions of Caspase8had no statistical significance compared with the control group (P>0.05). The mRNA change of caspase9in5and25μmol/L DAPT treatment group increased with a dose-time dependent manner (P<0.05), but no statistical significance in1μmol/L group compared to the control group. The protein expression of Caspase9in1μmol/L group had no statistical significance compared with the control group. The protein expression of Notch1decreased significantly and performed a dose-time dependent manner, but the difference of the two groups treated with1μmol/L DAPT for48h and5μmol/L for24h had no statistical significance.Conclusions1. Notch signal pathway mediate the apoptosis of human gastric cancer cell line BGC-823.2. Downregulation of Notchl can promote apoptosis of human gastric cancer cell line BGC-823.3. The Notch-mediated apoptosis mechanism may relate to mitochondrial pathway in human gastric cancer cell line BGC-823. not death receptor pathway.