Studies On The Chemical Constituents Of Rhaponticum Carthamoides (Willd.) Iljin

Rhaponticum carthamoides (Willd.) Iljin. is a plant of Compositae family, distributing only in northwest of Xinjiang province(e.g Ta Cheng, Fu Yun) in China, and Mongolia, Siberia of Russia and Middle Asia in worldwide. In Xinjiang, the roots of Rh. carthamoides is generally used as a analeptic agent to treat elderly sexual insufficiency, weariness, impotence and cardiovascular disease and so on. Some former USSR researchers used to hold the view that the phytoecdysteroids from Rh. carthamoides had the adaptive effects which is pretty similar to traditional Chinese medicine gingsen, medofenoxate and pilose antler. Present pharmacological research indicates that Rh. carthamoides has many biological activities such as antihypertensive, lipid lowering and body-strenthening effects.From the 70% ethanol extract of roots of Rh. carthamoides,22 compouds were obtained by means of solvent partition, repeated column chromatography on silica gel, Sephadex LH-20 column, open ODS column, polyamide column, P-TLC and recrystallization. On the basis of physico-chemical properties and spectroscopic analysis, 20 compounds of them were identified as follows:4-hydroxybenzoic acid (1),2, 4-dihydroxybenzoic acid (2),3,4-dihydroxybenzoic acid (3),3-hydroxy-4-methoxyl-benzoic acid (4), cinnamic acid (5), kaempferol (6), isorhamnetin (7), wogonin (8), quercetin (9), quercetin-5-O-β-D-glucopyranoside (10), 3-O-methyl-quercetin-5-O-β-D-glucopyranoside (11), ursolic acid (12), euscaphic acid (13),β-ecdysterone (14), buplerol (15), isolappaol A (16), benzyl-O-β-D-glucopyranoside (17), syringin (18),β-sitosterol(19), daucosterol (20).Among them, compounds 2,4,5,8,10,13,15,16,17 were obtained from this genus for the first time and compounds 12,18 were obtained from Rh. carthamoides for the first time.