| Biochemical analyzer is the instrument commonly used in clinical examination, which has become one of the essential equipments in medical institutions. By measuring biochemical indicator using blood and other body fluid, it can provide doctor-s and chemical inspectors with testing data accurately and rapidly. Spectrophotometer is an important component in biochemical analyzer, whose performance will affect the accuracy of the entire system. The leve of spectrophotometer directly influence the accuracy of biochemical analyzer.The spectrophotometer introduces the technology of back-dividing-light,which was designed with concave holographic grating. The concave holographic grating divides composite light with biochemical indicators into12beams of light signal at the same time, and illuminate to the surface of array detector. Then the signal is magnified through AD conversion, and finally collected and processed. The spectrophotometer detects some beams of light signal at the same time, which can realize fast measurement, and improve the accuracy and stability of the biochemical analyzer. The paper studied the design of spectrophotometer in the system and applied it to the biochemical analyzer.This thesis was divided into four parts. The first section analyzed the principle and error of spectrophotometry and introduced the structure and performance index of spectrophotometer. The second section expounded the aberration theory of flat-field concave holographic grating in the view of optical path difference, advanced optimization of grating’s aberration, and introduced workmanship of flat-field concave holographic grating. The third section analyzed the design procedure of spectrophotometer, include the design of preposition-optical system, optical splitting system, amplifier and AD conversion, introduced the process of collecting, processing and display the data. The last section stated the detections of spectrophotometer’s performance index, which match the requirement, and can be employed in biochemistry detection. |