| Objective To observe the curve of plasma uric acid metabolism after beer ingestion and To investigate the effect of acarbose administered orally before beer ingestion on the plasma concentration changes of uric acid, creatinine, glucose, insulin, IL-113and FEUA.Methods After an overnight fast,10healthy males completely voided their urine at7am. Urine samples were then collected every hour for a total of six times (period1,2,3,4,5and6), and blood was drawn at the mid-point of each one-hour collection period. After the first urine samples were collected, beer (15ml/kg body weight) was ingested orally within5minutes. Two weeks later, the second experiment was performed with the same protocol, except that acarbose (100mg) was administered at the beginning of beer ingestion. The concentrations of uric acid and creatinine in plasma and urine were measured. Plasma glucose, insulin, and IL-1β were also measured in period1,2,3and4(namely fasting,0.5h,1.5h,2.5h after beer ingestion)Results (1)The plasma concentration of uric acid increased respectively by1.09-fold (301.30±44.01vs276.70±35.37, P<0.05),1.31-fold (363.80±66.75vs276.70±35.37, P<0.01),1.22-fold (336.50±48.64vs276.70±35.37, P<0.01),1.11-fold (307.90±39.42vs276.70±35.37, P<0.05) at0.5hour,1.5hour,2.5hour,3.5hour after beer ingestion alone and increased respectively by1.12-fold (319.14±53.71vs285.68±45.86, P<0.01),1.16-fold (329.84±56.01vs285.68±45.86, P<0.01),1.09-fold (311.10±52.89vs285.68±45.86, P<0.05) at0.5hour,1.5hour,2.5hour in combination group. The plasma concentration of uric acid did not increase significantly at3.5hour (297.99±42.49vs285.68±45.86, P>0.05). The difference was not statistically significant between two groups (P=0.65).(2) The plasma concentration of glucose increased than fasting at0.5h (5.91±0.65vs4.66±0.50, P0.01) in beer group and at0.5h (5.59±0.75vs4.49±0.40, P<0.01),1.5h (5.01±0.56vs4.49±0.40, P<0.05) in combination group. Two groups had no statistical significances (P=0.87).(3) The plasma concentration of insulin increased than fasting at0.5h (26.89±16.55vs4.86±1.95,12.56±6.61vs5.43±2.12, P<0.01),1.5h (9.68±5.78vs4.86±1.95,8.12±3.34vs5.43±2.12, P<0.05) in two groups. The area under the insulin curve (34.18±20.62vs21.95±8.99, P<0.05) and the rising level of peak value (22.03±15.50vs7.13±5.32, P<0.01) between two groups were statistically significant.(4) The FEUA of period4, period5, period6was decreased than period2in two groups, but there were no differences between two groups (P=0.153).(5) The plasma concentrations of creatinine and IL-1β did not change significantly in and between two groups (P>0.05)Conclusion The plasma concentration of uric acid increased to maximum at1.5hour after beer ingestion at a standard of15ml/kg,1.31-fold of fasting concentration, and returned to baseline gradually after3.5hour. The urate-lower effect of acarbose was not investigated in this study in the case of young males drinking beer at a standard of15ml/kg and without eating. This may be associated with the subjects age, the number of cases and lack of enough carbohydrate intake. One-time right amount of consumption of beer had no effect on creatinine metabolism and IL-Iβ level. |
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