Sword Auricle Grass Screening Of Active Ingredients And The Experimental Research Of The Hepatitis B Role

Objective:(1) To extract each component of the Hedyotis lance (Jianyeercao).(2)To observe the protective effect of Hedyotis lance’s extracts on the models of CC14induced acute liver injury in mice, and filter out the extract has a protective effect. Methods:(1) To use water extraction, then get the Hedyotis lance aqueous extract; To reflux extraction Hedyotis lance with80%ethanol, and finally get the alcohol total extractings. According to the polarity of solvent, we extracted the alcohol total extractings with petroleum ether, chloroform, Ethyl acetate, n-butyl alcohol in proper order, and then get the corresponding parts.(2) To observe the protective effect of Hedyotis lance’s each extract on acute liver injury in mice. The model of CC14induced acute liver injury in mice with the method of intraperitoneal injection, finally the indexes of liver, spleen and thymus were observed. The colorimetric assay was used to measure the activities of alanine aminotransferase (ALT) and aspartate aminotransferase(AST) in mouse blood serum, and the activity of superoxide dismutase (SOD), Glutathione-SH(GSH) and Glutathione peroxidase(GSH-PX), the content of malondialdehyde (MDA) in liver homogenate were determined. Results:(1) Hedyotis lance’s aqueous extract and petroleum ether extract, chloroform extract, ethyl acetate extract, n-butyl alcohol extract were collected.(2) Hedyotis lance’s aqueous extract and ethyl acetate extract could obviously reduce the weights of liver, spleen and thymus; the activities of AST, ALT and the content of MDA could be decreased; the activities of SOD, GSH and GSH-PX could be increased(P<0.01或P<0.05). Conclusion:Hedyotis lance’s aqueous extract and ethyl acetate extract have protective effect on acute hepatic injuried mice. They are worth studying further. Objective:To observe the protective effect of Hedyotis lance aqueous extract and ethyl acetate extract on acute chemical liver injury in mice and the mechanism of action.Methods:The models of AP and D-GalN induced acute liver injury in mice with the method of intraperitoneal injection were used to study the protective effects of Hedyotis lance aqueous extract and ethyl acetate extract. Finally the indexes of liver, spleen and thymus were observed. The colorimetric assay was used to measure the activities of ALT, AST and alkaline phosphatase (AKP), the content of albumin (Alb), the ability of total antioxidant capacity (T-AOC) in mouse serum, and the activity of SOD, GSH and GSH-PX, the content of MDA in liver homogenate were determined. Hematoxylineosin (HE) stain was used to examine the degree of hepatic injury. Results:Hedyotis lance aqueous extract and ethyl acetate extract could obviously reduce the weights of liver, spleen and thymus; the activities of AST, ALT, AKP and the content of MDA could be decreased; the content of Alb, the ability of T-AOC, and the activities of SOD, GSH and GSH-PX could be increased(P<0.01或P<0.05). The degree of hepatic injury could be lessened. Conclusion:Hedyotis lance aqueous extract and ethyl acetate extract have protective effect on acute chemical liver injury. The mechanism may be related to attenuating free radical and inhibiting the effect on lipid peroxidation. Objective:To study the effect of Hedyotis lance’s aqueous extract and ethyl acetate extract against CCl4-induced liver fibrosis in rats and its mechanism. Methods:Wistar rats were established by intragastric administration (i.g.) of 50%CCl4two times a week for8weeks.80wistar rats of hepatic fibrosis group that confirmed by the pathological inspection were divided into8groups randomly. All rats were treated with corresponding drugs for six consecutive weeks by i.g., one time a day.24hours after the last administration of drugs, all rats were killed. The activities of ALT, AST and the content of Hyaluronic acid (HA) in serum were measured; the rats’indexes of liver, spleen and thymus were observed; and the activity of SOD, GSH-PX, the content of MDA, Hydroxyproline (Hyp) in liver homogenate were determined. Hematoxylineosin (HE) stain was used to examine the degree of hepatic fibrosis in rats. Results: Compared with the model group, Hedyotis lance’s aqueous extract and ethyl acetate extract could obviously reduce the weights of liver, spleen and thymus; the activities of AST, ALT, the content of HA in serum, and the content of MDA, Hyp in liver homogenate could be decreased; the activities of SOD, and GSH-PX in liver homogenate could be increased(P<0.01或P<0.05). The pathological results of HE staining showed that, Compared with the model group, the rank of hepatic fibrosis in rats of Hedyotis lance’s aqueous extract and ethyl acetate extract and positive control group was significantly lower(P<0.01或P<0.05). Conclusion:Hedyotis lance’s aqueous extract and ethyl acetate extract might have certain curative effect on CCl4-induced liver fibrosis in rats, its mechanism may be related to attenuating free radical, inhibiting the lipid peroxidation, reducing the collagen synthesis and the deposition, decreasing the sedimentation of ECM and increasing the degradation of ECM. Objective:To observe the toxicity of Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum on HepG2.2.15cell; to observe the inhibitive effect of Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum against HBsAg and HBeAg produced by HepG2.2.15cells. Methods:(1) The inhibition ratio, TC50and TC0of Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum to the HepG2.2.15cells transfected with HBV gene was determined by MTT assay.(2) To study the effect of different concentrations of Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum on HepG2.2.15cells, after4d and8d of incubation, the culture medium was collected for determining the levels of HBsAg, HBeAg by ELISA. Results:(1) Different concentrations of Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum had little cellulotoxicity on HepG2.2.15cells.(2) Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum in different concentration could suppress HBsAg and HBeAg expressions. The anti-HBV effect was dose-dependent and time-dependent; all TI>2. Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum are effective and low toxic anti-HBV drugs. Conclusion:Hedyotis lance’s aqueous extract and ethyl acetate extract and their medicated serum can inhibit significantly HBV in vitro. This study provided the evidence for the further research of its pharmacological action and clinical use.