| Objective1We intuitionally observe the blood rheology characteristics on the patients with acute coronary syndrome(ACS) after PCI over one year by Micro Channel array Flow Analyzer(MC-FAN),combined with other platelet rheological indices.2We discuss the relativity between the blood transiting time in MC-FAN (MC-FAN TT)and the traditional Chinese medicine blood stasis syndrome scoring, the indices of platelet function, inflammatory factors, echocardiographic to add new rheological visualization detection basis for blood stasis syndrome scoring, and provide reference for the treatment in insisting activating blood and dissolving stasis.Materials and Methods1This study bring into74patients with ACS after PCI for one to three years, without drug intervention, recording the blood stasis syndrome scoring and filling the CRF, making the PAgT, PAdT, and detecting the P-selection, PDGF-BB, vWF, hs-CRP, IL-6, BNP, echocardiographic and so on. Then, we use MC-FAN HR300to detect the transiting time about the blood pass through the model body capillaries. At the same time, we make21healthy controls for comparing, and research the characteristics about the patients and the relativity between MC-FAN TT and the Traditional Chinese medicine blood stasis syndrome scoring, the indices of platelet function, inflammatory factors, echocardiographic.2Statistical analysis:We use the SPSS18.0statistical software to dispose all the data,and P<0.05stands for the differences have statistical significance.Under the condition that all the data have been used normality test,the data conforming to normal distribution expressed by x±s,and the data not conforming to normal distribution expressed by median.We use t-test, F-test, Wilcoxon and Kruskal-Wallis H test to analyse the relationship among the groups in measurement data,and use Chi-square test in enumeration data.To analyse the relativity between MC-FAN TT and the grade of Traditional Chinese medicine blood stasis syndrome, platelet rheology, inflammatory factors, echocardiographic, we use correlation analysis and multivariate linear regression analysis.Results1The comparison between groups of ACS after PCI and health matchedCross-sectional study, the blood stasis syndrome scoring in ACS after PCI group is obviously higher than health matched group(P<0.01).From the comparison in10μ lTT,30μ lTT,60μlTT,100μlTT of MC-FAN between the two groups, we know the TT of ACS after PCI group is longer than the one of health matched group, and the differences have statistics significance(P<0.01). PAgT, PAdT, P-selection, PDGF-BB in ACS after PCI group is higher than the ones in health matched group, and the differences have statistics significance(P<0.01).The detection results of vWF,inflammatory factor,platelet parameters(include platelet count, platelet hematocrit, Platelet distribution width, mean platelet volume,Large platelet ratio),BNP between the two groups have no differences(P>0.05).2The correlation between the MC-FAN and the blood stasis syndrome graded, each test indices in ACS after interventional patients10μlTT,30μlTT have positive correlations with the blood stasis syndrome scoring(r=0.676,P=0.000; r=0.315,P=0.013).10μlTT,30μlTT,60μlTT have positive correlations with P-selection(r=0.601, P=0.000; r=0.334, P=0.009; r=0.354, p=0.029);10μlTT,30μlTT have positive correlations with PDGF-BB(r=0.432, P=0.000; r=0.260, P=0.043);10μlTT,30μlTT,60μlTT have positive correlations with vWF(r=0.517, P=0.000; r=0.277, P=0.031; r=0.364, P=0.025);10μlTT,60μlTT,100μl TT have positive correlations with PAgT(r=0.314, P=0.030; r=0.527, P=0.007; r=0.756, P=0.030);10μlTT,100μlTT have positive correlations with PAdT(r=0.503, P=0.000; r=0.815, P=0.014).30μlTT has negative correlation with LVDD(r=-0.272, P=0.039).60μlTT has negative correlation with LVEF(r=-0.326, P=0.049).10μlTT,60μlTT have positive correlations with hs-CRP(r=0.300, P=0.011; r=0.482, P=0.003).30μlTT has positive correlation with PCT(r=0.268, P=0.038).The blood stasis syndrome scoring has the most significant correlation with10μlTT. LVDD has the most significant correlation with30μlTT.PAgT has the most significant correlation with60μlTT. PAdT has the most significant correlation with100μlTT.Other test results have no correlation with MC-FANTT.3The detection results comparison of different factorsThrough grouping according to single or double antiplatelet therapy, different number of merger disease, different time after PCI, and comparing if the observational results have difference, we know all groups’results have no differences(P>0.05). So single or double antiplatelet therapy, number of merger disease and different time after PCI(1to3years about postoperative) have no affection on each index.Conclusion1We know from the MC-FAN and platelet function index detection that the transiting time of ACS patients after PCI is long, the ability of deformation decreased in RBC,the adhesion and aggregation increased in WBC and platelet, at the same time, blood stasis syndrome scoring and platelet rheology are all abnormal, compared with the healthy control group. MC-FAN can intuitionally observe the patients’chenge of blood rheology and objectively evaluate the condition of the patients’blood rheology.2Compared with the healthy control group, the indices of vWF, IL-6, hs-CRP, BNP, PLT, PDW, PCT,MPV, P-LCR in ACS after PCI have no differences.The three factors about single or dual antiplatelet treatment, merger disease number, length of postoperation time(after PCI one to three years) have no influence on the detection results.3Parts of MC-FAN TT have positive correlations with blood stasis syndrome graded, the results of PAgT, PAdT, P-selection, PDGF-BB, vWF, PCT, hs-CRP, and they have negative correlation with LVDD and LVEF. MC-FAN reflects the patients’state of blood stasis objectively and intuitionally.It can be used as an objective lab test basis for blood stasis syndrome scoring,and it can provide reference for insisting activating blood and dissolving stasis treatment, combining with the assess of blood stasis syndrome scoring. |
PDF Full Text Request