| 【Objective】Based on better clinical effect of massage therapy on sciatic nerve injury, this study is to research the recovery on both sensory and motor function after massage therapy. Meanwhile, through the research on changes of GAP-43expression in DRG, the study can explore massage’s approach and central mechanism on the treatment of sciatic nerve injury.【Methods】Taking SD rats as experimental animals to build clamping injured model, the study used massage simulator on UB37, BL57and GB34. And also, solar-thermal pain threshold resistance and inclined plate test is used from a behavioral point of view to explore the recovery on sensory and motor function after therapy. Through observing the variation of DRG, the spinal marrow, sciatic nerve and the HE of gastrocnemius, the promotion of therapy’s effect on neuronal regeneration is sought on aspect of morphology. Through the research on changes of GAP-43expression in DRG and gastrocnemius muscles, approach and mechanism is revealed by massage therapy on sciatic nerve injury.【Results】1. EthologySeven days after made the model, results of inclined plate group declined which has statistical significance compared with normal group. Ten days after massage therapy, curative effect of inclined plate group is better than sham operation group, model group and control group. It means that massage therapy can improve gastrocnemius muscle for the sciatic nerve injury and promote the recovery of motor function.Seven days after made the model, solar-thermal pain threshold resistance in model group is increased and it has statistical significance compared with normal group. Ten days after massage therapy, solar-thermal pain threshold resistance in therapy group is obviously lower than model group and higher than normal group. It has statistical significance compared with model group. It showed massage therapy can improve pain sensitivity for the sciatic nerve injury and promote the recovery of sensory function.2. PathologyUnder normal circumstances, the neuron of DRG HE stain is visibly round and cytoplasm is eosinophilic and the cell volume is a bit larger. Cell nucleus is basophilic, staining light, large and round. Cell nucleolus is clear with satellite cells enveloping. HE in the spinal marrow is visible and cell nucleus is located in the central cells. Nissl body distributed uniformly of the cell bodies.After sciatic nerve injured, part of DRG neurons appeared that chromatin dissolved. Neurons have different degrees of necrosis. Some cells were swelled and dissolved with some deformation. Cytoplasm staining wasn’t even with form size differ. HE staining of the spinal cord cell swelled with degeneration phenomenon. The nucleus moved toward the edge and nissl body dissolved and disappeared. It showed that after sciatic nerve has injured, DRG and motor neuron cell of spinal cord ventral-horn died.After massage therapy, DRG neuron is in the shape of gradual rules, mostly round. Cell is swelled and deformation phenomenon is not obvious. Part of ventral-horn nerve nucleus moved toward the edge and part of the nucleus is located in the central. Nissl body dissolved, cells array is better than model group and model control group in integrity and direction. Massage therapy can promote the recovery of both sensory and motor neuron after the sciatic nerve injured.3. Growth associated proteinGAP-43in normal group and sham operation group has some expression in DRG and gastrocnemius muscles. Positive neurons cytoplasm appeared light brown, nucleus appeared pale blue. After seven days of sciatic nerve injured, GAP-43expression from model group and model control group in DRG and gastrocnemius muscles is deepen staining than normal group. AOD was increased, where there are differences. After ten days of massage therapy, AOD of GAP-43from model group and model control group in DRG and gastrocnemius muscles has a bit increase than normal group. AOD of GAP-43in DRG and AOD of GAP-43in gastrocnemius from massage therapy group significantly increased, staining extent and scope significantly better than model group. The expression is more obvious than the other four groups which has statistical significance.[Conclusion]Massage therapy can promote pain sensitivity of sciatic nerve injured rats, it also can increase gastrocnemius muscle’s recovery rate. Both sensory and motor function is enhanced on the sciatic nerve injury. It effected on sensory neurons in DRG and motor neurons in the spinal marrow. The mechanism is for the promotion of GAP-43expression in both DRG and gastrocnemius muscles’growth-related proteins to promote the regeneration of injured nerves, and provide a good microcnvironment for the injured nerve. |
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