Effect Of Active-peptide Supplementation On Calpain MRNA Expression Of Skeletal Muscle

OBJECTIVE:This study was designed by observing the complementary peptide of Calpain-1, Calpain-2gene expression to explore the role of the peptide in the prevention of skeletal muscle protein degradation, meanwhile expected to interpret the physiological mechanism of skeletal muscle micro-injury in the sub-cellular level to some degreeMETHODS:8-week male SD rats were prepared for6weeks treadmill exercise. The rats were randomly divided into10groups of10each. They were divided into control group, exercise0,24,48h group, exercise0,24,48h+peptide group, exercise0,24,48h+soyprotein group. The levels of serum T. IGF-1levels were mesured by Radioimmunoassay and the skeletal muscle Calpain-1, Calpain-2mRNA levels were mesured by Real Time-PCR.RESULTS:(1)After exercise, serum IGF-1of peptide exercise had no significant difference with exercise group and soy protein exercise group; as time goes on, before and after exercise, there is no significant difference (P<0.05)(2)24h after exercise, serum T of peptide exercise group had significant difference with exercise group(P<0.05);as time goes on, non-significant difference were found among groups.(3)Immediately after exercise, vastus intermedialis muscle Calpain-1gene expression levels had significant difference with exercise group and soy protein exercise group(P<0.05);as time goes on, significant difference were found among groups(P<0.05).24h after exercise, vastus intermedialis muscle Calpain-2gene expression levels had significant difference with soy protein exercise group(P<0.05), as time goes on, non-significant difference were found among groups.(4)24h after exercise, gastrocnemius Calpain-1gene expression levels had significant difference with exercise group (P<0.05)48h after exercise, gastrocnemius Calpain-1gene expression levels had significant difference with soy protein exercise group (P<0.05), as time goes on, non-significant difference were found among groups;Immediately after exercise and48h after exercise, gastrocnemius Calpain-2gene expression levels had significant difference with protein exercise group(P<0.01);as time goes on, significant difference were found among groups(P<0.01).CONCLUSION:(1)Bioactive peptides and soy proteins are able to reduce serum IGF-1and serum T recovery time after exercise. Bioactive peptides is better than soy protein.(2)After exercise, the skeletal muscle CANP-1, CANP-2gene expression showed different changes. CANP-1gene expression was downregulated with time, and CANP-2gene was upregulated.(3)Bioactive peptides may have a protective effect for skeletal muscle during exercise making the CANP-1gene expression downregulate delay, delayed the starting time of self-protection mechanism. Soy protein may have a role in the inflammatory response after exercise.(4)After exercise, CANP-1gene expression continued to be regulated down, while the gastrocnemius CANP-1, CANP-2gene expression increased first and then decreased, which may be related to the type of muscle fiber.