| In recent years, emergency pollution of drinking water incidence showed a rising trend andthe safety of drinking water face serious challenge. Effectively monitoring the abrupt change ofharmful chemicals such as biological toxins in drinking water has drawn much attention forresearch of emergency management by government all over the word.The objective of this thesis was to develop a novel method to determine emergencycontamination of chemical toxins ɑ-solanine in drinking water by using flow injection analysiswith luminol chemiluminescence （FLA-CL）. The main contents include:（1） Evalution of thecorrelation between the electric conductivity, pH value and ɑ-solanine pollution in drinkingwater;（2） Determination of ɑ-solanine by luminol-potassium ferricyanide CL in drinking water;（3） Effect of different solvent systems on extraction of ɑ-solanine and determination of optimumcondition for ɑ-solanine extraction;（4） The effect of storage conditions （temperature, light, time）on the content of ɑ-solanine;（5） Comparative study of three methods colorimetric method, highperformance liquid chromatography and flow injection chemiluminescence method for thedetermination of the ɑ-solanine content. The main results were listed as follows:1. The electric conductivity and pH value changed proportionally to the concentration ofɑ-solanine. Compared with the control samples, there was no significant change in electricconductivity and pH value under the presence of1×10-4mg/mL ɑ-solanine; When theconcentration of ɑ-solanine reached up to5×10-4mg/mL, electrical conductivity and pH valuechanged obviously.2. According to the reduction effect of ɑ-solanine by CL reaction between lumionl andpotassium ferricyanide by a new method for rapid determination of ɑ-solanine with flowinjection analysis technology was established. The linear range was8.0×10-4～1.0×10-1mg/mLwith the detection limit of6.09×10-5mg/mL.3. Comparison of three methods for extraction of ɑ-solanine from germinated green potatosby treatments of95%ethanol,5%acetic acid and ethanol-acetic acid mixed solvent,respectively, showed that the ethanol-acetic acid mixed solvent extraction rate and recoverywas relatively higher, which reached100%and92.0%, respectively. Results from one way testand orthogonal experiment showed that impact factors for efficiency of ɑ-solanine extraction are in ratio of solid-liquid> extraction temperature> extraction time> ultrasonic frequency,extraction time35min, ultrasonic frequency60Hz, extraction temperature60℃, and ratio ofsolid-liquid1:4（m/v）.4. Storage conditions （temperature, light, time） results showed that storage under lightinduces production of ɑ-solanine more than storage in the dark and high temperature and longstorage time can promote production of ɑ-solanine. When potato stored in25℃with a light, thecontent of ɑ-solanine reached10.53mg/100g after15days.5. Comparative study of colorimetric method, high performance liquid chromatography andflow injection chemiluminescence methods for determination of the ɑ-solanine content showedthat high performance liquid chromatography and flow injection chemiluminescence had higherrecovery of ɑ-solanine, which reached95.0%and90.0%.In conclusion, combination of flow-injection with chemiluminescence can be a potentialon-line method for determination of the emergency pollution of chemical toxins such asɑ-solanine in drinking water. |
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