On-line Determination Of Deoxynivalenol Sudden Pollution In Drinking Water By Flow Injection Chemiluminescence And Emergency Warning System

Urban drinking water connects millions of households.Once it was suddenly polluted,it would endanger the health of millions of citizens,even their lives.Online monitoring and timely warning of sudden pollution of drinking water was an important issue in the study of drinking water safety.In this paper,the flow injection chemiluminescence(CL)on-line monitoring method for deoxynivalenol(DON)was established by using the effect of DON on luminol-hydrogen peroxide-tween 80 system.The main contents of the study include:(1)enrichment and characterization of DON;(2)response surface methodology to optimize the detection of DON in Luminol-hydrogen peroxide-Tween 80 system;(3)on-line monitoring of DON by flow injection chemiluminescence to set a dynamic toxicity threshold;(4)removal of DON in drinking water.The main results are as follows:1.DON can be easily yielded from Fusarium graminearum with its optimum growth conditions.The yield of DON was impacted by carbon,nitrogen and humidity.The yield of DON were incresead when 3% sucrose,0.3% ammonium sulfate and 50% water were added.2.The flow injection CL on-line dectection of DON was eatablished based on the enhancement effect of DON on the CL reaction of luminol-hydrogen peroxide in alkaline medium.The influences of various experimental factors such as sodium hydroxide,luminol,hydrogen peroxide,Tween 80 concentrations were investigated.Under the optimum conditions,the enhanced CL intensity was linearly related to the concentration of DON in the range of 0.001~4.000 mg/L with a detection limit(3σ)of 0.001 mg/L.The precision was calculated by analyzing sample containing 0.2 mg/L DON(n=11)and the relative standard deviation(RSD)was 1.89%.This technique method can be applied to the on-line detection of sudden contamination of DON.3.The dynamic range of safety threshold was set up for early warning of DON sudden pollution.The dynamic toxicity threshold of DON was calculated as X±5SD(X: the average of the data monitored in the previous hour,SD: the standard deviation of the data monitored in the previous hour).This range was used to evaluate indicators of drinking water safety.4.Powdered activated carborn(PAC)was used to effectively remove DON sudden pollution in drinking water.DON in drinking water was absorbed by PAC.The adsorption effect was determined by the concentration of PAC and adsorption time.When DON in drinking water exceeded 4 times of the standard,the removal rate of PAC could reach 82.25% within 50 minutes.This method can be applied to remove DON in drinking water.