Studies On The Application Of CE In Pharmaceutical And Biology Analysis

Chapter1:This paper introduced the development of the CE technology, the basic principle, the instrument structure, the separation mode, the injection techniques, the detection method and hyphenated modes. The application of CE in the western drugs, the chiral drugs, the traditional Chinese herbs and the bodily fluid samples were summarized. The main content and significance of this paper were introduced, too.Chapter2:In this chapter, a rapid and simple capillary zone electrophoresis(CZE) method has been successfully developed for the determination of homocysteine(Hcy) and reduced glutathione (rGSH) in the human serum.20mmol/LTris-H3PO4、pH=2.1as the optimal back ground electrolyte, applied voltage25kV, working temperature25℃and detection wavelength220nm. The linear range for Hey and GSH were200μM～1000μM and100μM～600μM with the correlation coefficient (r) of0.9988and0.9949. The limit of detection(S/N=3) for homocysteine and glutathione were8.63μmol/L and14.21μmol/L. The relative standard deviation (RSD) of the peak area of homocysteine and glutathione were1.29%～3.60%,2.18%～4.20%,1.17%～4.35%and1.99%～4.91%for intra-day and inter-day analyses, respectively. Furthermore, satisfactory results were obtained for recovery and repeatability. The proposed method, quick and sensitive, which was successfully applied to analyze the homocysteine and glutathione in the human serum.Chapter3:In this paper, to establish a rapid and simple capillary zone electrophoresis to detect the galanthamine hydrobromide in pharmaceutical and human serum. The best experimental conditions:50mmol/L NaH2PO4-H3PO4、pH=2.5as the background electrolyte, separation voltage is15kV, the temperature is25℃, detection wavelength is289nm. The experimental results show that:the galanthamine hydrobromide has a good linear relationship in the range of0.015mg/mL-0.160mg/mL with the correlation coefficient (r) of0.9989, the limit of detection (S/N=3) was2.57×10-5mg/mL. The relative standard deviation (RSD) of intra-day(n=3) and inter-day(n=3) of the migration time and peak area were0.10%～0.22%,0.13%～1.10%and0.14%～0.34%,0.76%-1.63%, respectively. The good results for precision and repeatability were obtained. The method can be applied to detect the galanthamine hydrobromide in pharmaceutical and human serum, quickly and simple.Chapter4:In this paper, A capillary electrophoresis with diode array detection for the determination of lovastatin and simvastatin in pharmaceutical and human serum. An uncoated fused capillary(62cm x75μm) was used.25mmol/L NaH2PO4-H3PO4,pH=9.2as the background electrolyte. The relative standard deviation (RSD) of the migration time and peak area of the intra-day for lovastatin were0.26%～1.66%、0.89%～2.89%, simvastatin were0.11%～1.19%、1.63%～2.17%; inter-day for lovastatin were0.90%-1.59%、1.84%～3.09%; simvastatin were0.36%～1.31%、1.98%～3.96%, respectively. There is a good linear relationship between the peak areas and concentration of the lovastatin and simvastatin in the range of0.030mg/mL‖0.273mg/mL and0.066mg/mL-0.591mg/mL, with the correlation coefficients were0.9949and0.9929. The limit of detection (S/N=3) were4.35X10-5mg/mL and7.44×10-5mg/mL, respectively. The proposed method can be used to detect the prescription drugs and human serum, and provide a simple and workable method for quality control of the drugs.