Study On The Sulfamethazine And Aspirin Molecularly Imprinted Polymer Microspheres

Molecularly imprinted polymer （MIP） has specific recognition ability to target molecule,it isprepared according to molecular imprinting technology(MIT).In this paper,molecularlyimprinted polymer microspheres（MIPs） is synthesized with sulfamethazine and asprin astemplate molecular via precipitation polymerization.And the synthesis conditions, mechanismof molecular imprinting and binding ability of MIPs are studied by a series of experiments.Molecularly imprinted polymer (MIP) microspheres are prepared via precipitation polymerizationwith sulfamethazine as template. Equilibrium adsorption experiment is used to discuss the bindingperformance and the results reveal high bingding affinity and selectivity to sulfamethazine.Scatchardanalysis shows that the MIPs have one class of binding sites is observed for the imprinted microspheresbinding to the template with the maximum apparent binding constant31.87μmol/g respectively.Molecularly imprinted polymer (MIP) microspheres are prepared via bulk polymerization andprecipitation polymerization with asprin as template.The optimization of the preparation conditions:Theinteraction between template molecule and functional monomer is inspected by ultraviolet spectrumanalysis, and species and mixing ratio of monomers are optimized.Using different rate of templatemolecule、functional monomer and cross-linking agent,a series of MIPs are prepared,optimum ratio isobtained by study on adsorption performance of this series of MIPs to template molecule.The equilibrium adsorption experiment was used to discuss the binding performance and the resultsshowed that the bingding capabilities of the imprinted polymer was higher than the non-imprintedpolymer microspheres(NIPs) to temlate. The selective adsorption experiment’s results showed that theselective recognition ability of the imprinted polymer microspheres is good.The imprinted polymermicrospheres which is prepared via precipitation polymerization binding to the template with the maximumapparent binding constant31.87μmol/g and the equilibrium dissociation constant0.5mg/mL is better thanthe imprinted polymer microspheres which is prepared via bulk polymerization.