Study On The Metal Elements And Polysaccharides Of Malania Oleifera

In this paper, the determination contents of metal elements, extraction and purification of polysaccharides from malania oleifera and the antioxidant activity of malania oleifera polysaccharides were studied systemicly. All the results were showed below:1. Samples of seeds and shells of malania oleifera were analyzed by HNO3-HClO4digestion and inductively coupled plasma-atomic emission spectrometry (ICP-AES) for contents of seven metal elements including Al, Ca, Fe, K, Mg, Mn and Zn. The results showed that the seeds contained Al18.65μg·g-1, Ca192.6μg·g-1, Fe9.946μg·g-1, Mn25.55μg·g-1, Zn23.46μg·g-1, K12.12mg·g-1, and Mg2.259mg·g-1; the shells contained Al10.75μg·g-1, Ca351.7μg·g-1,K3.435mg·g-1Mg289.3μg·g-1,Mn11.42μg·g-1and Zn9.987μg·g-1.he detection limit of each elements was from0.0012to0.1609μg·g-1, the relative standard deviation (RSD, n=5) was from1.04%to7.58%, and the recovery ratio of spiked sample was from97.49%to109.6%. The method had a good accuracy and precision.2. The traditional water extraction method was studied. Based on single factor experiment, the orthogonal experiments were carried out. The descending order of factors affecting the extraction of polysaccharides from malania oleifera were extraction temperature> extraction times> liquid materials ratio> extraction time. And the optimization process was obtain as below:the malania oleifera was extraction for three times, liquid materials ratio was1:20, extraction time was3h and extraction temperature was90℃. In optional conditions, the extraction rate of polysaccharide from malania oleifera was8.98%.3. Then, the paper studied the elimination of protein by the Sevage method from polysaccharides. The results showed that polysaccharide retention was76.97%, and protein removal rate was46.55%. The decolorization of polysaccharide was also studied by dynmic adsorption polyamide. The best condition for decolorization were determined as follows:the amount of polyamide was15g, elution volume was1column volume, and flow rate was2.0mL·min-1. Under the this condition, polysaccharide retention and decolourization ratio were77.6%and87.8%.4. Via removing protein, decoloring and removing small molecules polysaccharides of malania oleifera were separated into two ingredient(PSM-A and PSM-B) by DEAE cellulose column chromatography. Then we found that PSM-A consists of one component, and PSM-B consists of two components by Sephadex G-100column chromatography.5. The prior to purification of the polysaccharide was palm red solid, non-starch polysaccharides and had a polysaccharide physical connectivity. It can react with with ninhydrin and was positive indicating that the Prior to purification of the polysaccharide contained protein or nucleic acid substances. Purified polysaccharide had good affects in protein removing and decolorization by UV spectrum scanning. PSM-A and PSM-B were pink powder. IR analysis shows that the crude polysaccharide, PSM-A and PSM-B were alpha-type glycosidic bond pyran polysaccharide.6. The antioxidant activity of prior to purification of the polysaccharide, PSM-A and PSM-B was discussed. The results showed that they had the ability of scavenging hydroxyl free radical and DPPH·radical.