Study On Cheqiancao Granule

Herba plantaginis is very famous Traditional Chinese Medicine recorded first in "shen nong ben cao jing", and is listed as the top grade. Seeds are used as medicine. Herba plantaginis is the dried whole plant as medicine in"mingyibielu"."bencaojingjizhu"says many herba plantaginis grow on roadside."bencaotujing"says jianghu, Huai Dian, near Beijing, the North has everywhere". Then they have been recorded in chinese pharmacopeiaas as Plantago asiatica L.and Plantago depressa Willd.Plantain granules is composed of plantain herb pieces by water extraction, purification, concentration, drying and granulating made formula granules. Herba plantaginis for its various pharmacological actions such as Heat diuretic, expectorant, cooling blood detoxification. For the treatment of edema oliguria, hot and pain, damp diarrhea, phlegm heat cough, hemoptysis and bleeding, boils. But because the traditional Chinese Formula granule is more excellent in the clinic than the traditional Chinese medicine in the aspect of decoction and taking. Herba plantaginis dispensing granule has stable efficacy and is convenient to take especially in comparison with the traditional herbal infusion. the use of modern preparation techniques, combined with pharmacodynamic study, develop lling plantain Pieces of bulk drugs used as medicine standard of quality control plantain recipe particles, in order to ensure the clinicalthe safety and efficacy of the drug, the paper its quality standards, the fingerprints of its pharmacodynamic equivalence study, the relevant state departments to develop a formula particles national standards to provide a scientific basis and reference standards.In the medicine quality standard study, according to the Chinese pharmacopoeia2010edition a Herba plantaginis item, to the character, identification, inspection, extract,content determination in the detection, by comparing the three group of Herba plantaginis herbs, found in Herba plantaginis flavone and icariin content is higher. The average Plantamajoside content is1.33%, than pharmacopoeial requirements, so take the Herba plantaginis as epimedium Dispensing Granule’s ingredients.In the preparation technology study, combined with Herba plantaginis contains composition to the physical and chemical properties, draw up a preparation process route, using orthogonal test method to have a comprehensive evaluation, and choosing the best preparation technology conditions for:10times the amount of water decoction, three times, each time for1hour, making each decoction of liquid medicine mixed, filtering, making the filtrate to relative density1.05liquid extract by reducing pressure, and powdering it in the oven which is in Spray-dried into extract powder, In proportion to join dextrin (extract powder:dextrin=1:1), blending, with70%ethanol do wetting agent, and on the14th mesh to extruding particles, in the last, tidying the particles.In the quality standard study, the epimedium formula grain’s quality control of various indicators having been studied systemly, about the character, provisions of this product for tan to brown, gas micro, taste slightly bitter;about the jianbie,having a thin-layer chromatography for Plantamajoside identifing a new chromatographic condition about the checking, having examining the granule size, moisture, melted sex and charge difference according to the granules of Chinese pharmacopoeia2010edition. focus on research to identify, extract and determination of the content determination, using high performance liquid chromatography (HPLC) method for the Plantamajoside’s content, using high performance liquid chromatography (HPLC) method for the Plantamajoside content, the product preparation methods, chromatography conditions, system suitability test and methodology investigation, based on batches of samples results tentative:This product1g carplantain recipe particles containing Plantamajoside glycosides not less than2mg.In the formula granules fingerprint study, studying the fingerprint by using the method of HPLC, including the product preparation methods, chromatographic conditions, determined to acetonitrile0.1%formic acid in water gradient elution chromatography conditions determine the basic four peaks in the chromatograms for fingerprint feature plantain recipe particles, method integrity features, stability was validated that basically meet the requirements. Using "computer aided similarity evaluation system2004A "for10batches of epimedium formula granules fingerprint has carried on the full spectrum of the similarity evaluation, the results,fingerprint similarity coefficient of each batch of samples are greater than0.95. Determination of the index components fingerprint combination can improve the level of internal quality control plantain recipe particles. Specification, the front of the vehicle controllable Herba plantaginis recipe granular product quality standards system is developed on the basis of the experimentalThrough the above a series of studies, preparation process is reasonable, suitable for actual industrial production,the established quality standard of Herba plantaginis formula granules and fingerprint method are simple, fast, standardized, scientific, and the result is stable, reliable, these have given a comprehensive and reliable gist for the quality control of Herba plantaginis and its products. determination of water-loading in mice administered5-hour urine volume using mouse metabolic cages was used to compare therapeutic action. To compare formula granule with decoction pieces standard broth of cheqiancao and liquored cheqiancao in pharmacodynamics equivalence. Both cheqiancao formula granule and decoction pieces standard borth have a diuretic effect on mice. he pharmacological effects of both was no significant difference, a safe and effective stabilizing finished. Therefore, the clinical application of plantain recipe particles have a positive role in promoting.