Study On Preparation And Properties Of Immobilized Carrier

The main purpose of this research is to use silane coupling agent treatment technologyfor surface modification on glass fiber fabric, to prepare a suitable carrier for biologicalfixation. The carrier was used for lipase immobilization. Experimental samples for theE-glass fiber fabric, coupling agent is KH-550and KH-560. Adsorbed moisture and organicmatter was first removed by the heat treatment of glass fiber fabric, and then the number ofhydroxyl on the surface of fabric was increased under the pretreatment of NaOH solution. Atlast, it was dipped in silane coupling agent aqueous solution to prepare carrier for thebiological fixation. Water wettability, mechanical strength, surface functional groups, surfacemorphology and immobilized lipase rate were evaluated. Hydrophobic was enhanced withincreases of contact angle, the breaking strength was reduced; the surface roughness of thecarrier enlarged from electron microscope images, coupling agent was successfully receivedfrom infrared spectra. Significant effect of silane coupling agent treatment was shown as theincrease the rate of lipase immobilization.Effect of factors of silane treatment on contact angle in the processing of carrier was alsostudied. With increase of silane concentration, contact angle first increased then decreased;With increase of dipping time, contact angle first increased then tended towards stability. Thecondition of silane was selected as concentration3%(volume fraction) and dipping time10min. Meanwhile, the surface roughness was enlarged, the rate of lipase immobilization washigher than before.Results of the study indicate that the method of using silane coupling agent on glassfiber fabric for surface modification to prepare immobilized carrier was viable, performanceof the carrier was improved.