Study On The Preparation Of Antibacterial Agents From Swine Blood Hydrolyzed By Trypsin And The Development Of Hand Sanitizer

In China, the swine resource utilization is very low, less than10%, mainlybecause of swine hemoglobin in color and smell, limiting its application. Only a smallpart as food supplies, to be processed into blood meal or fermented blood meal as afeed additive. The use of swine ’s blood can be used for other biochemical reagentspharmaceutical production and development of peptides and peptide drugs and foodadditives. Deep processing and utilization of swine hemoglobin has become one ofthe focus areas of the current animal protein.In this paper as raw swine investigated factors on enzymatic extraction swinelimited impact of antimicrobial agents, such as the type of enzyme hydrolysis time,enzyme dosage, reaction temperature, etc., in the various factors of a single after thefactors investigated by orthogonal test method is limited to swine swine enzymaticextraction conditions were optimized antimicrobial agents, the optimal enzymaticprocess: choose trypsin digestion time16h, hydrolysis temperature35℃, enzymeThe amount0.8mg/ml (IU=250U/g). Three parallel test inhibitory potency(inhibition zone) of25.41mm,24.06mm,25.77mm, the average value of25.08mm,the optimal value of the orthogonal experiment comparing its relative error is0.98%.Antifungal activity of antimicrobial agents swine was studied by the minimuminhibitory concentration of the test, and the minimum bactericidal concentration oftest Test Time-Killer conclusion: the five kinds of antimicrobial agents swine standardstrains (Escherichia coli ATCC25922, gold aureus ATCC25923, Pseudomonasaeruginosa ATCC27853, Streptococcus pneumoniae ATCC49619, Candida albicansATCC10231) MIC values were125μg mL-1,125μg mL-1,125μg mL-1,62.5μg mL-1,1000μg mL-1; MBC values were125μg mL-1,125μg mL-1,250μg mL-1,62.5μg mL-1,2000μg mL-1.Against5strains TimeKiller test sample of E. coliATCC25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosaATCC27853, Streptococcus pneumoniae ATCC49619kill better average kill ratewithin10minutes90%or more, and the effect of killing Candida albicans in general,the number of colonies still maintain a certain level after10minutes. Antibacterial agent with bactericidal ingredients swine to the matrix carbomer,glycerin as the main materials, prepared hand sanitizer has a good bactericidal effect.Test response surface optimized to hand sanitizer formulations swine antibacterialagent as an active ingredient:50%swine antimicrobial agent content, the content of4%glycerol,0.34%was prepared under the conditions of the content of carbomerhand sanitizer its liquid antibacterial activity (inhibition zone) can reach the maximum29.81mm. Three parallel test antibacterial activity (inhibition zone) were29.73mm,29.52mm,29.80mm, the relative error of the predicted value of1.20%.Of swine antibacterial agent with bactericidal ingredients hand sanitizer physicaland chemical indicators, microbial indicators and stability of the inquiry, the handsanitizer the indicators are in line with the provisions of the special GB19877.1-2005hand sanitizer national standards after its stability monitoring a year to prove its goodbactericidal properties, stable, with a shelf life of more than1year.