Preparation And Stability Of Paprika Red Niosome

Paprika red is a kind of natural pigmentwith high color value, non-toxic, and nutritional value, but the low water-solubility limits its application in the fields of food,pharmaceutical, and cosmetic. The optimal conditions of encapsulating paprika red into niosomes werestudied using single factor analysis method and response surface analysis method. The stability of the pigment encapsulated in niosome and not encapsulated were compared, which included the light illumination, thermal treatment and oxidative stress,and the physical and chemical characters of noisome were also analyzed.1. The preparation of paprika red niosome. The content of paprika red, ultrasonic time and HLB of surfactants were the major factors for noisome preparation, and the effects of paprika red content and ultrasonic time were significant. The optimum conditions were that the content of paprika red was0.05g, cholesterol was0.2g, HLB of surfactant was8.2(span80was0.27g, tween20was0.13g) and the ultrasonic time was24min.Under above conditions, niosome encapsulation rate reached82.4%and niosomes had good water-solubility.2. The stability of paprika red niosome. Under light irradiation,niosome had limited protective effect on paprika red,and even accelerated its degradation in the later period of degradation. Niosome had protective effect on paprika red in dark,resulting in the degradation rate decreasing by10.5%.Thermal stability experiments exhibited niosome had little protective effect on paprika red in50℃but the degradation rate of freeze-dried powder in cold environment was significantly lower than that of paprika red niosome solution,indicating freeze-dried powder of paprika red niosome had good cold storage solubility. In oxidoreduction experiments,the degradation rate of paprika red niosome was higher than that of control after H2O2was added The degradation rate of niosome became lower with VE, added which indicating VE can reduce the rate of degradation. 3. The physicochemical property of paprika red niosome. Niosome distributed equally and particle size was between100-500nm scanned by TEM.DSC analysis showed that the glass transition temperature of niosome was39.4℃in blank vesicle, but not found in paprika red niosome.Paprika red niosome had no significant increasing in thermostability.FTIR of paprika red niosome showed paprika red successful embedded itself in niosome, and paprika red and the components of niosome had no chemical reaction.