Cloning And Characterization Of Salt Tolerant Relevant Genes GmVP, GmCHR And Gmusp1in Soybean(Glycine Max(L.)Merr.)

Saline-alkali soil is an important type of soil, nearly half of all irrigated lands are affected bysalinity. Most of this salinity and all of the sodicity are natural form. However, a significantproportion of recently cultivated agricultural land has become saline because of land cultivation orirrigation.It causes a serious threat to cultivation crops. As an important food and economic crop,Soybean is badly influenced by saline-alkali soil in development, yield and quality. Carrying out theresearch of soybean salt resistance genes excavation and genetic engineering are crucial, for thescarcity of the soybean salt resistance varieties resource. Based on the previous research, we clonedand characterizated three salt resistance relevant genes, preliminary analyzed the function andpotential breeding application value.We cloned three new salt tolerance relevant genes from soybean through the selection of saltinduced suppression subtraction library (SSH), named respectively GmVP, GmCHR, GmUsp1.Sequence homology analysis showed that three genes belong to H~+-pyrophosphatase (H~+-PPase),NADPH-dependent Aldo-keto Reductases, Universal Stress Protein(UspA).Sub-cellular localization result showed GmVP located on the membrane structure,overexpreesion GmVP could significatively improve the relative root growth under the salt stress，and improve the salt tolerance of the hairy roots. The Na~+, K~+ion content of GmVP transgenichairy roots is different from the CK after treatment of50mmol/L NaCl,100mmol/L KCl or1mmol/L KCl. Overexpression GmVP gene could help to suppress Na~+absorption, adjust K~+/Na~+balance of trangenic hariy roots, maintain the ions homeostasis under the salt stress. And it alsoincreased the activity of SOD, POD,CAT and reduced the MDA content. Overexpreesion GmCHRalso could improve the hairy roots relative growth under the salt stress, and improve the salttolerance of the hairy roots.The preliminary research result confirmed that overexpress GmPIP can improve the salttolerance of soybean hairy roots and the Superroot-derived Lotus corniculatus plant. For the furtherresearch of this gene, we constructed GmPIP expression vector and transformed GmPIP to soybeancultivar Jilinxiaoli NO.1, via the Agrobacterium tumefaciems-mediated soybean cotyledonary-nodetransformation system. Up to now,3T0transgenic plants were confirmed by160mg/L glufosinatescreening and molecular analysis, which would be useful for further studies of the characterizationand function of GmPIP and soybean transgenic salt resistance breeding.