The Injury Mechanism Of Deoxynivalenol,Zearalenone And Co-infection To Liver And Kidney Of Rat

270healthy adult female mice, were randomly assigned to9groups:control group (10%methanol), DON infected group(test team I and test team II, dose of DON were1^2.5mg/kgBW)、ZEA infected group(test teamⅢand test teamIV, dose of ZEA were20、30mg/kgBW)、combined infected group(test team Ⅴ-Ⅷ, DON1.5mg/kgBW and ZEA20mg/kgBW, DON1.5mg/kgBW and ZEA30mg/kgBW, DON2.5mg/kgBW and ZEA20mg/kgBW, DON2.5mg/kgBW and ZEA30mg/kgBW); two factors and three levels: DON concentration levels of0,1.5,2.5mg/kgBW, ZEA levels of0,20,30mg/kg BW, were injected by peritoneal cavity every24h for4d successively. Samples were harvested at different time points (3d,5d,8d, and12d), then observe changes in liver and kidney pathology, and determine serum biochemical indicators, liver and kidney antioxidant indicators as well as liver and kidney apoptosis rate, to study the effects of DON, ZEA and the combined effects of these two toxins on rat liver and kidney. The results were as follows:(1) The combined toxins exposed mice all could cause damage to mouse liver and kidney, showing that liver cells and kidney cells underwent degeneration and necrosis, and the histopathologic damage to mouse liver and kidney in the combined high concentration group (DON2.5mg/kgBW+ZEA30mg/kgBW) are the most serious.(2) On3d and5d, the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum increased, the content of total protein (TP) decreased, and creatinine (Cr) and urea nitrogen (BUN) levels increased. Compared combined toxins exposure group with single toxin exposure group, the difference was significant or extremely significant (P<0.05or P<0.01). Among them, functional parameter in blood of liver and kidney in the combined high concentration group (DON2.5mg/kgBW+ZEA30mg/kgBW) are affected most seriously.(3) On3d and5d, the activity of superoxide dismutase (SOD) in liver and kidney tissue decreased, malondialdehyde (MDA) content increased, tissue inhibiting capacity to hydroxyl free decreased, nitric oxide (NO) content and activity of nitric oxide synthase (NOS) increased. Compared combined toxins exposure group with single toxin exposure group, the difference was significant or extremely significant (P<0.05or P<0.01), and apparente dose-effect relationship between different concentration groups was showed. Among them, the oxidative damage of liver and kidney in the combined high concentration group (DON2.5mg/kgBW+ZEA30mg/kgBW) are the most serious.(4) On3d and5d, the apoptosis rate of liver and kidney increased, significantly or extremely significantly different from control group (P<0.05or P<0.01); compared combined toxins exposure group with single toxin exposure group, the difference was significant or extremely significant (P<0.05or P<0.01), and apparente dose-effect relationship between different concentration groups was showed. Among them, the apoptosis rate of liver and kidney in the combined high concentration group (DON2.5mg/kgBW+ZEA30mg/kgBW) are affected most seriously.(5) Toxic effect of DON and ZEA obviously decreased on8d and12d, but combined toxins exposure group still showed relatively strong toxicity, and every index notablely differed from that of control group. The results showed that:DON, ZEA alone and the combined toxins exposed mice all could cause mouse liver and kidney tissue pathologically damaged, antioxidant capacity decreased and apoptosis rate increased, eventually, result in liver and kidney functions impaired; Deuto-acrylic additive effect was the main interaction effect between DON and ZEA.